Abstract
The mouse skin tumor promoter phorbol-12-myristate-13-acetate (PMA) is a potent inflammatory agent that not only elicits the infiltration of neutrophils, monocytes and lymphocytes to the site of application (1) but also induces human neutrophils and monocytes, but not lymphocytes, to release clastogenic factor(s) (CF) (2). CF are characterized as lipophilic (ethylacetate extractable) substances of relatively low molecular weight (<10 kDa) whose formation is mediated by superoxide anions and whose activity is inhibited by Cn superoxide dismutase (SOD), free-radical scavengers and antagonists of arachidonic acid (AA) metabolism (3). A correlation between CF formation and lipid peroxidation has been demonstrated (4). These properties of CF are suggestive of free-radical chain reactions which can give rise to lipid peroxides and possibly account for the formation and/or action of CF.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
A. Viaje, T. Slaga, M. Wigler and I. Weinstein, Effect of anti-inflammatory agents on mouse skin tumor promotion, epidermal DNA synthesis, phorbol ester-induced cellular proliferation and production of plasminogen activator. Cancer Res. 37, 1530–1536 (1977).
I. Emerit and P. Cerutti, Clastogenic action of tumor promoter phorbol-12-myristate-13-acetate in mixed human leukocyte cultures. Carcinogenesis 4, 1313–1316 (1983).
I. Emerit and P. Cerutti, Tumor promoter phorbol-12-myristate-13-ace-tate induces a clastogenic factor in human lymphocytes. Proc. Natl. Acad. Sci. U.S.A. 79, 7509–7513 (1982).
I. Emerit, S. Khan and P. Cerutti, Treatment of lymphocyte cultures with a hypoxanthine-xanthine oxidase system induces the formation of transferable clastogenic material. J. Free Radical Biol. Med. 1, 51–57 (1985).
P. Cerutti, Prooxidant states and tumor promotion. Science 22, 375–381 (1985).
I. Emerit, A. Levy and P. Cerutti, Suppression of tumor promoter phorbol myristate acetate-induced chromosome breakage by antioxidants and inhibitors of arachidonic acid metabolism. Mutat. Res. 110, 327–335 (1983).
Y. Keisari, I. Geva, E. Flescher, H. Golden and G. Lavie, Stimulation of human monocyte oxidative burst and related cytotoxicity by tumor-promoting and non-tumor-promoting diterpene esters, indole alkaloids and poly-acetate-type agents. Int. J. Cancer 36, 467–472 (1985).
F. Chilton, J. Ellis, S. Olson and R. Wykle, 1-O-alkyl-2-arachidonyl-sn-glycerol-3-phosphocholine. J.Biol. Chem. 259, 12014–12019 (1984).
T. Eling, B. Tainer, A. Ally and R. Warnock, Separation of archidonic acid metabolites by high pressure liquid chromatography. Methods Enzymol. 86, 511–517 (1982).
A. Benedetti, R. Fulceri and M. Comporti, Inhibition of calcium sequestration activity of liver microsomes by 4-hydroxyalkenals originating from the peroxidation of liver microsomal lipids. Biochim. Biophys. Acta- 793, 793, 489–493 (1984).
G. Camussi, M. Aglietta, R. Coda, F. Bussolino, W. Piacibello and C. Tetta, Release of platelet-activating factor (PAF) and histamine. Immunology 42, 191–199 (1982).
T. Yasaka, L. Boxer and R. Baehner, Monocyte aggregation and superoxide anion release in response to formyl-methionyl-leucyl-phenylalanine (FMLP) and platelet-activating factor (PAF). J. Immunol. 128, 1939–1944 (1982).
F. Chilton, J. O’Flaherty, C. Walsh, M. Thomas, R. Wykler, L. de Chatelet and B. Waite, Platelet-activating factor. J. Biol. Chem. 257, 5402–5407 (1982).
W. Kozumbo, D. Muehlematter, A. Jorg, I. Emerit and P. Cerutti, Phorbol ester-induced formation of clastogenic factor from human monocytes. Carcinogenesis 8, 521–526 (1987).
T. Ochi and P. Cerutti, Clastogenic action of hydroperoxy-5,8,11,13-icosatetraenoic acids on the mouse embryo fibroblasts C3H/10T2. Proc. Natl. Acad. Sci. U.S.A. 84, 990–994 (1987).
J. Lewis, T. Hamilton and D. Adams, The effect of macrophage development on the release of reactive oxygen intermediates and lipid oxidation products, and their ability to induce oxidative DNA damage in mammalian cells. Carcinogenesis 7, 813–818 (1986).
S. Lesko, R. Lorentzen and P. Ts’o, Role of superoxide in deoxyribonucleic acid strand scission. Biochemistry 19, 3023–3028 (1980).
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1987 Plenum Press, New York
About this chapter
Cite this chapter
Kozumbo, W., Muehlematter, D., Ochi, T., Cerutti, P. (1987). The Role of Active Oxygen and the Metabolism of Arachidonic Acid in the Formation of Clastogenic Factor by Human Monocytes. In: Cerutti, P.A., Nygaard, O.F., Simic, M.G. (eds) Anticarcinogenesis and Radiation Protection. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-6462-1_8
Download citation
DOI: https://doi.org/10.1007/978-1-4615-6462-1_8
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4615-6464-5
Online ISBN: 978-1-4615-6462-1
eBook Packages: Springer Book Archive