Hydroxyl Free Radical Mediated Strand Breaks in PBR322 Plasmid DNA

Schneider, E.; Watson, J. J.; Floyd, R. A.

doi:10.1007/978-1-4615-6462-1_14

E. Schneider⁴,
J. J. Watson⁴ &
R. A. Floyd⁴

112 Accesses

Abstract

Oxygen free radicals react with and cause damage to DNA. Hydroxyl free radicals (·OH) add to DNA to yield several products, one of which is 8-hydroxydeoxyguanine (1). Digestion of DNA to the nucleoside level yields 8-hydroxydeoxyguanosine (8-OHdG) which can be very sensitively quantitated (to 20 femtomoles) by HPLC-electrochemical detection (2,3). The potential significance of the 8-OHdG adduct in living systems has been demonstrated by its presence in the DNA of human granulocytes following TPA-stimulated oxidative damage (3). The amount of 8-OHdG present in the granulocyte DNA was decreased by the addition of superoxide dismutase and/or catalase, thus emphasizing that oxygen free radical intermediates are the responsible agents (3).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution

Chapter: USD 29.95; Price excludes VAT (USA)

eBook: USD 84.99; Price excludes VAT (USA)

Softcover Book: USD 109.99; Price excludes VAT (USA)

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

References

H. Kasai and S. Nishimura, Hydroxylation of deoxyguanosine at the C-8 position by ascorbic acid and other reducing agents. Nucleic Acids Res. 12, 2137–2145 (1984).
Article PubMed CAS Google Scholar
R.A. Floyd, J.J. Vatson, P.K. Wong, D.H. Altmiller, R.C. Rickard, Hydroxyl free radical adduct of deoxyguanosine: sensitive detection and mechanisms of formation. Free Radical Res. Commun. 1, 163–172 (1986).
CAS Google Scholar
R.A. Floyd, J.J. Watson, J. Harris, M. West, and P.K. Wong, Formation of 8-hydroxyguanosine, hydroxyl free radical adduct of DNA in granulocytes exposed to the tumor promoter, tetradeconylphoboloutate. Biochem. Biophys. Res. Commun. 137, 841–846 (1986).
Article PubMed CAS Google Scholar
K. Brawn and I. Fridovich, DNA strand scission by enzymatically generated oxygen radicals. Arch. Biochem. Biophys. 206, 414–419 (1981).
Article PubMed CAS Google Scholar
P. Geek and I. Nasz, Concentrated, digestible DNA after hydroxylapetite chromatography with cetylpyridinium bromide precipitation. Anal. Biochem. 135, 264–268 (1983).
Article Google Scholar

Download references

Author information

Authors and Affiliations

Molecular Toxicology Research Group, Oklahoma Medical Research Foundation, 731040, Oklahoma City, Oklahoma, USA
E. Schneider, J. J. Watson & R. A. Floyd

Authors

E. Schneider
View author publications
You can also search for this author in PubMed Google Scholar
J. J. Watson
View author publications
You can also search for this author in PubMed Google Scholar
R. A. Floyd
View author publications
You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

Swiss Institute for Experimental Cancer Research, Lausanne, Switzerland
Peter A. Cerutti
Case Western Reserve University, Cleveland, Ohio, USA
Oddvar F. Nygaard
National Bureau of Standards, Gaithersburg, Maryland, USA
Michael G. Simic

Rights and permissions

Reprints and permissions

Copyright information

About this chapter

Cite this chapter

Schneider, E., Watson, J.J., Floyd, R.A. (1987). Hydroxyl Free Radical Mediated Strand Breaks in PBR322 Plasmid DNA. In: Cerutti, P.A., Nygaard, O.F., Simic, M.G. (eds) Anticarcinogenesis and Radiation Protection. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-6462-1_14

Download citation

DOI: https://doi.org/10.1007/978-1-4615-6462-1_14
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4615-6464-5
Online ISBN: 978-1-4615-6462-1
eBook Packages: Springer Book Archive

Publish with us

Policies and ethics