Abstract
The surface protein S of transmissible gastroenteritis virus (TGEV) has a sialic acid binding activity that enables the virus to agglutinate erythrocytes. A protocol is described that has been successfully applied to the isolation of hemgglutination-defective mutants. The potential of these mutants for the characterization of the sialic acid-binding site and the function of the binding activity is discussed.
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© 1998 Springer Science+Business Media New York
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Krempl, C., Ballesteros, M.L., Enjuanes, L., Herrler, G. (1998). Isolation of Hemagglutination-Defective Mutants for the Analysis of the Sialic Acid Binding Activity of Transmissible Gastroenteritis Virus. In: Enjuanes, L., Siddell, S.G., Spaan, W. (eds) Coronaviruses and Arteriviruses. Advances in Experimental Medicine and Biology, vol 440. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5331-1_73
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DOI: https://doi.org/10.1007/978-1-4615-5331-1_73
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