Abstract
The positive-strand transcripts as well as negative-strand transcripts of mouse hepatitis virus (MHV) defective interfering (DI) RNA, when introduced into MHV-infected cells, re-sulted in DI RNA replication and accumulation. The leader sequence of the majority of DI RNAs that accumulated from the expression of negative-strand DI RNA transcripts with no extra non-MHV nucleotides at the 3’ end switched to that of helper virus, whereas this leader sequence switching did not occur in most of the positive-strand DI RNAs that accumulated from the expressed negative-strand DI RNA transcripts with extra non-MHV nucleotides at the 3’ end. These data demonstrated that the extra 4 nucleotides at the 3’-end of negative-strand DI RNA transcripts affected leader sequence switching on DI RNA, and indicated that the leader switching probably occurred during positive-strand DI RNA synthesis.
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References
Chang, R.-Y., Krishnan, R. and Brian, David, 1996, The UCUAAAC promoter motif is not required for high frequency leader recombination in bovine coronavirus defective interfering RNA, J. Virol. 70:2720–2729.
Fuerst, T.R., Niles, E.G., Studier, F.W., and Moss, B., 1986, Eukaryotic transient-expression system based on recombination vaccinia virus that synthesizes bacteriophage T7 polymerase, Proc. Natl. Acad. Sci. USA 83:8122–8126.
Jeong, Y.S., and Makino, S., 1992, Mechanism of coronavirus transcription:duration of primary transcription initiation activity and effect of subgenomic RNA transcription on RNA replication, J. Virol. 66:3339–3346.
Lai, M.M.C., and Stohlman, S.A., 1978, RNA of mouse hepatitis virus, J. Virol. 26:236–242.
Lai, M.M.C., Baric, R.S., Brayton, RR. and Stohlman, S.A., 1984, Characterization of leader RNA sequences on the virion and mRNA of mouse hepatitis virus, a cytoplasmic virus, Proc. Natl. Acad. Sci. USA 81:3626–3630.
Lai, M.M.C., Brayton, P.R., Armen, R.C., Patton, CD., Pugh, C, and Stohlman, S.A., 1981, Mouse hepatitis virus A59: mRNA structure and genetic localization of the sequence divergence from hepatotropic strain MHV-3, J. Virol. 39:823–834.
Lee, H.-J., Shieh, C.-K., Gorbalenya, A.E., Eugene, E.V., La Monica, N., Tuler, J., Bagdzhadzhyan, A., and Lai, M.M.C., 1991, The complete sequence (22 kilobases) of murine Coronavirus genel encoding the putative proteases and RNA polymerase, Virology 180:567–582.
Leibowitz, J.L., Wilhelmsen, K.C., and Bond, C.W., 1981, The virus-specific intracellular RNA species of two murine coronaviruses: MHV-A59 and MHV-JHM, Virology 114:39–51.
Makino, S., and Lai, M.M.C., 1989, High frequency leader sequence switching during coronavirus defective interfering RNA replication, J. Virol. 63:5285–5292.
Makino, S., Taguchi, F., Hirano, N., and Fujiwara, K., 1984, Analysis of genomic and intracellular viral RNAs of small plaque mutants of mouse hepatitis virus, JHM strain, Virology 139:138–151.
Makino, S., Shieh, C.-K., Soe, L.H., Baker, S., and Lai, M.M.C., 1988, Primary structure and translation of a defective interfering RNA of murine Coronavirus, Virology 166:550–560.
Sawicki, S.G., and Sawicki, D.L., 1990, Coronavirus transcription: subgenomic mouse hepatitis virus replicative intermediates function in RNA synthesis, J. Virol. 64:1050–1056.
Sawicki, S.G., and Sawicki, D.L., 1986, Coronavirus minus-strand mRNA synthesis and effect of cyclohexamide on Coronavirus RNA synthesis, J. Virol. 57:328–334.
Schaad, M.C., and Baric, R.S., 1994, Genetics of mouse hepatitis virus transcription: evidence that subgenomic negative strands are functional templates, J. Virol. 68:8169–8179.
Sethna, P.B., Hofmann, M.A., and Brian, D.A., 1991, Minus-strand copies of replicating coronavirus mRNA con-tain antileaders, J. Virol. 65:320–325.
Sethna, P.B., Hung, S.-L., and Brian, D.A., 1989, Coronavirus subgenomic minus-strand RNAs and the potential for mRNA replicons, Proc. Natl. Acad. Sci. USA 86:5626–5630.
Spaan, W., Delius, H., Skinner, M., Armstrong, J., Rottier, P., Smeekens, S., van der Zeijst, B.A.M., and Siddell, S.G., 1983, Coronavirus mRNA synthesis involves fusion of non- contiguous sequences, EMBO J. 2:1939–1944.
Woo, K., Joo, M., Narayanan, K., Kim, K.H., and Makino, S., 1997, Murine Coronavirus packaging signal confers packaging to nonviral RNA, J. Virol. 71:824–827.
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Banerjee, S., Makino, S. (1998). Studies of Murine Coronavirus DI RNA Replication from Negative-Strand Transcripts. In: Enjuanes, L., Siddell, S.G., Spaan, W. (eds) Coronaviruses and Arteriviruses. Advances in Experimental Medicine and Biology, vol 440. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-5331-1_30
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DOI: https://doi.org/10.1007/978-1-4615-5331-1_30
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