Abstract
Efforts to develop respiratory and enteric vaccines focus on the efficient delivery of protective antigens to the mucosal lymphoid tissue. One approach is to use recombinant viral vectors, i.e. apathogenic viruses with a tropism for mucosal surfaces and genetically manipulated to express the protective antigens. Adenoviruses, particularly the human adenovirus type 5 (Ad5), are promising candidates for use as vectors.
Chapter PDF
Similar content being viewed by others
Keywords
- Recombinant Adenovirus
- Protective Antigen
- Transmissible Gastroenteritis Virus
- Polymerase Chain Reaction Process
- Recombinant Viral Vector
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
Callebaut, P., Debouck, P., and Pensaert, M., 1982, Enzyme linked immunosorbent assay for the detection of the coronavirus-like agent and its antibodies in pigs with porcine epidemic diarrhea. Vet. Microbiol. 7: 295.
Correa, I., Gebauer, F., Bullido, M., Suné, C., Baay, M.F.D., Zwaagstra, K.A., Posthumus, W.P.A., Lenstra, J.A., Enjuanes, L., 1990, Localization of antigenic sites of the E2 glycoprotein of transmissible gastroenteritis coronavirus. J. Gen. Viro1.71: 271.
Graham, F.L., and Prevec, L., 1991, Manipulation of adenovirus vectors. In: “Methods in Molecular Biology” Murray, E.J., ed., Humana Press, 109.
Pensaert, M., Callebaut, P., and Vergote, J., 1986, Isolation of porcine respiratory, non-enteric coronavirus related to transmissible gastroenteritis. Vet. Quart. 8:257.
Prevec, L., Campbell, J.B., Christie, B.S., Belbeck, L., and Graham, F.L., 1990, A recombinant human adenovirus vaccine against rabies. J. Infect. Dis. 161: 27.
Rasschaert, D., Duarte, M., and Laude, H., 1990, Porcine respiratory coronavirus differs from transmissible gastroenteritis virus by a few genomic deletions. J. Gen. Virol. 71:2599.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1994 Springer Science+Business Media New York
About this chapter
Cite this chapter
Callebaut, P., Pensaert, M., Enjuanes, L. (1994). Construction of a Recombinant Adenovirus for the Expression of the Glycoprotein S Antigen of Porcine Respiratory Coronavirus. In: Laude, H., Vautherot, JF. (eds) Coronaviruses. Advances in Experimental Medicine and Biology, vol 342. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-2996-5_74
Download citation
DOI: https://doi.org/10.1007/978-1-4615-2996-5_74
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-6305-7
Online ISBN: 978-1-4615-2996-5
eBook Packages: Springer Book Archive