Abstract
Cis-acting signals located in the 3’-UTR of the viral genome were first identified by deletion analyses of MHV defective interfering (DI)-RNAs (Kim, Jeong, and Makino, 1993; Lin and Lai, 1993). The cis-acting signals for the synthesis of minus-strand RNA are contained within the last 55 nucleotides (nt) plus the poly (A) tail (Lin, Liao, and Lai, 1994). Our lab has previously shown that host cell proteins specifically bind to two distinct sites within the MHV 3’UTR (Yu and Leibowitz, 1995b). In the current work, we show that the RNA-protein (RNP) complex formed within the last 42 nt of the genomic RNA contains four proteins of molecular mass 90, 70, 58 and 40 kDa and identify the 90 kDa protein as mitochondrial aconitase.
Chapter PDF
Similar content being viewed by others
Keywords
- Ferric Ammonium Citrate
- Mouse Hepatitis Virus
- Iron Regulatory Protein
- Defective Interfere
- Mitochondrial Aconitase
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
References
Kennedy, M. C., Mende-Mueller, L., Blondin, G. A., and Beinert, H. (1992). Purification and characterization of cytosolic aconitase from beef liver and its relationship to the iron-responsive element binding protein. Proc Natl Acad Sci USA 89,(24), 11730–11734.
Kim, H. Y., LaVaute, T., Iwai, K., Klausner, R. D., and Rouault, T. A. (1996). Identification of a conserved and functional iron-responsive element in the 5′-untranslated region of mammalian mitochondrial aconitase. J. Biol Chem. 271, 24226–24230.
Kim, Y.-N., Jeong, Y. S., and Makino, S. (1993). Analysis of cis-acting sequences essential for Coronavirus defective interfering RNA replication. Virology 197, 53–63.
Klausner, R. D., Rouault, T. A., and Harford, J. B. (1993). Regulating the fate of mRNA: the control of cellular iron metabolism. Cell 72,(1), 19–28.
Leibowitz, J. L., Wilhelmsen, K. C., and Bond, C. W. (1981). The virus-specific intracellular RNA species of two murine coronaviruses: MHV-A59 and MHV-JHM. Virology 114, 39–51.
Lin, Y., Liao, C., and Lai, M. M. C. (1994). Identification of the cis-acting signal for minusstrand RNA synthesis of a murine Coronavirus: implications for the role of minus-strand RNA in RNA replication and transcription. J. Virol. 68(12), 8131–8140.
Lin, Y.-J., and Lai, M. M. C. (1993). Deletion mapping of a mouse hepatitis virus defective interfering RNA reveals the requirement of an internal and discontinous sequence for replication. J. Virol. 67, 6110–6118.
Liu, Q., Yu, W., and Leibowitz, J. L. (1997). A specific host cellular protein binding element near the 3′ end of mouse hepatitis virus genomic RNA. Virology 232, 74–85.
Soltys, B. J., and Gupta, R. S. (1999). Mitochondrial-matrix proteins at unexpected locations: are they exported? Trends Biochem. Sci. 24, 174–177.
Yu, W., and Leibowitz, J. L. (1995a). A conserved motif at the 3′ end of mouse hepatitis virus genomic RNA required for host protein binding and viral RNA replication. Virology 214, 128–138.
Yu, W., and Leibowitz, J. L. (1995b). Specific binding of host cellular proteins to multiple sites within the 3‱ end of mouse hepatitis virus genomic RNA. J. Virol. 69, 5033–5038.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Springer Science+Business Media New York
About this chapter
Cite this chapter
Nanda, S.K., Leibowitz, J.L. (2001). Mitochondrial Aconitase Binds to the 3’-UTR of Mouse Hepatitis Virus RNA. In: Lavi, E., Weiss, S.R., Hingley, S.T. (eds) The Nidoviruses. Advances in Experimental Medicine and Biology, vol 494. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1325-4_89
Download citation
DOI: https://doi.org/10.1007/978-1-4615-1325-4_89
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-5498-7
Online ISBN: 978-1-4615-1325-4
eBook Packages: Springer Book Archive