Abstract
In vitro production of tissues or tissue engineering is a promising approach to produce artificial tissues for regenerative medicine. There are at least three important components of tissue engineering, including stem cells, scaffolds and growth factors. This study aimed to produce cartilage tissues in vitro from culture and chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells (BMMSCs), induced by chondrogenesis medium, on biodegradable polycaprolactone (PCL) scaffolds. BMMSCs were isolated from rabbit bone marrow according to the standard protocol. The adherence, proliferation and differentiation of BMMSCs on scaffolds were investigated using two scaffold systems: PCL scaffolds and collagen-coated PCL (PCL/col) scaffolds. The results showed that BMMSCs could attach and grow on both PCL and PCL/col scaffolds. However, the adhesion efficacy of BMMSCs on the PCL/col scaffolds was significantly better than on PCL scaffolds. Under induced conditions, BMMSCs on PLC/col scaffolds showed increased aggrecan accumulation and upregulated expression of chondrogenesis-associated genes (e.g. collagen type II, collagen type I, aggrecan and collagen type X) after 3, 7, 21 and 28 days of induction. These in vitro cartilage tissues could form mature chondrocyte-like cells after they were grafted into rabbits. The results suggest that use of BMMSCs in combination with polycaprolactone scaffolds and chondrogenesis medium can be a way to form in vitro cartilage tissue.
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Abbreviations
- BMMSCs:
-
Bone marrow-derived mesenchymal stem cells
- CD:
-
Cluster of differentiation
- ECM:
-
Extracellular matrix
- GFP:
-
Green fluorescent protein
- MSC:
-
Mesenchymal stem cell
- PBS:
-
Phosphate buffer saline
- PCL:
-
Polycaprolactone
- PCL/col:
-
Collagen-coated PCL
- PCR:
-
Polymer chain reaction
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Acknowledgements
This research is funded by Vietnam National University Ho Chi Minh City (VNU-HCM) under grant numbers TX2017-18-02 and C2017-18-24/HĐ-KHCN and University of Science Ho Chi Minh City under grant number T2017-44.
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The authors declare that there is no conflict of interest regarding the publication of this paper.
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Dao, T.TT. et al. (2017). In Vitro Production of Cartilage Tissue from Rabbit Bone Marrow-Derived Mesenchymal Stem Cells and Polycaprolactone Scaffold. In: Pham, P. (eds) Tissue Engineering and Regenerative Medicine. Advances in Experimental Medicine and Biology(), vol 1084. Springer, Cham. https://doi.org/10.1007/5584_2017_133
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DOI: https://doi.org/10.1007/5584_2017_133
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