Protein Phosphatase Protocols

1. The Use of RNA Interference to Analyze Protein Phosphatase Function in Mammalian Cells

   • Iain Fraser, Wei Liu, Robert Rebres, Tamara Roach, Joelle Zavzavadjian, Leah Santat et al.

   Pages 261-286

2. Recognition of a PP2C Interaction Motif in Several Plant Protein Kinases

   • Niranjan Chakraborty, Masaru Ohta, Jian-Kang Zhu

   Pages 287-298

3. Use of Yeast Genetic Tools to Define Biological Roles of Novel Protein Phosphatases

   • Joaquín Ariño, Antonio Casamayor, Amparo Ruiz, Ivan Muñoz, Maribel Marquina

   Pages 299-307

4. Targeting of PP2C in Budding Yeast

   • Irene M. Ota, James Mapes

   Pages 309-322

5. Phosphatase Targets in TOR Signaling

   • Estela Jacinto

   Pages 323-334

6. Functional Characterization of Small CTD Phosphatases

   • Michele Yeo, Patrick S. Lin

   Pages 335-346

7. Genome-Scale Discovery and Characterization of Class-Specific Protein Sequences

   • David Kerk

   Pages 347-370

8. Yeast Substrate-Trapping System for Isolating Substrates of Protein Tyrosine Phosphatases

   • Masahide Fukada, Masaharu Noda

   Pages 371-381

9. Back Matter

   Pages 383-387

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Protein Phosphatase Protocols presents a broad range of protocols for the study of protein phosphatases, all written by experts and innovators from phosphatase laboratories around the world. This volume is a compendium of resources for the study of protein phosphatases and their potential as drug targets. Experimental methodologies are taken from proteomics, bioinformatics, genomics, biochemistry, RNAi, and genetics. Included protocols utilize several model organisms, including yeast, Drosophila, and various plant and human cells. Protein Phosphatase Protocols provides a concise introduction to protein phosphatase research, with particular attention to phosphatase families. Assays for identification, quantification, purification, and functional characterization are presented throughout in specific detail.

• Cell signaling networks
• Drosophilia
• Drug targets
• Phosphatase families
• Proteomics
• Termination
• Vivo
• Yeast
• biochemistry
• bioinformatics
• escherichia coli
• protein sequence
• saccharomyces cerevisiae

• Department of Biological Sciences, University of Calgary, Calgary, Canada

  Greg Moorhead

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