Plant Proteomics

1. Protein Identification Using Nano Liquid Chromatography-Tandem Mass Spectrometry

   • Luc Negroni

   Pages 235-248

2. Two-Dimensional Nanoflow Liquid Chromatography-Tandem Mass Spectrometry of Proteins Extracted from Rice Leaves and Roots

   • Linda Breci, Paul A. Haynes

   Pages 249-266

3. Separation, Identification, and Profiling of Membrane Proteins by GFC/IEC/SDS-PAGE and MALDI TOF MS

   • Wojciech Szponarski, Frédéric Delom, Nicolas Sommerer, Michel Rossignol, Rémy Gibrat

   Pages 267-278

4. The PROTICdb Database for 2-DE Proteomics

   • Olivier Langella, Michel Zivy, Johann Joets

   Pages 279-303

5. Identification of Phosphorylated Proteins

   • Maria V. Turkina, Alexander V. Vener

   Pages 305-316

6. Plant Proteomics and Glycosylation

   • Anne-Catherine Fitchette, Olivia Tran Dinh, Loïc Faye, Muriel Bardor

   Pages 317-342

7. Blue-Native Gel Electrophoresis for the Characterization of Protein Complexes in Plants

   • Jesco Heinemeyer, Dagmar Lewejohann, Hans-Peter Braun

   Pages 343-352

8. Electroelution of Intact Proteins from SDS-PAGE Gels and Their Subsequent MALDI-TOF MS Analysis

   • Zhentian Lei, Ajith Anand, Kirankumar S. Mysore, Lloyd W. Sumner

   Pages 353-363

9. Generation of Plant Protein Microarrays and Investigation of Antigen-Antibody Interactions

   • Birgit Kersten, Tanja Feilner

   Pages 365-378

10. Phosphorylation Studies Using Plant Protein Microarrays

    • Tanja Feilner, Birgit Kersten

    Pages 379-390

11. Back Matter

    Pages 391-399

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The aim of Plant Proteomics: Methods and Protocols is to present up-- date methods and protocols used by recognized scientists in the world of plant proteomics. If this world was a very small one twenty-five years ago when the first papers were published, it has since experienced exponential growth, and in most countries around the world there are laboratories working on plant proteomics. Two-dimensional gel electrophoresis is still the basic method used, but it has been improved greatly with IPG in the first dimension (Chapter 13) and with new detection methods with fluorochromes (Chapters 14 and 15). Signi- cant progress has been achieved in protein extraction, which is particularly difficult with plant tissues containing phenols, proteases, and other secondary metabolites that interfere with proteins. Standard procedures have been op- mized (Chapters 1 and 2) for peculiar tissues (Chapters 3, 4, and 5) and cellular compartments (Chapters 6 to 10). These methods rely on improvements made in the solubilization of proteins from membranes (Chapters 11 and 12). Mass spectrometry was a revolution that permitted the high throughput identifi- tion of proteins separated by 2D gels (Chapters 19 and 20) but also from blue native 1D gels (Chapters 27 and 28) despite the fact that Edman sequencing can still be useful (Chapter 18). Associated with other techniques such as 2DLC or LC of intact proteins, mass spectrometry also permits the identification of polypeptides from complexes (Chapters 21 and 22).

• Microarray
• Polyacrylamid-Gelelektrophorese
• Proteomics
• Seed
• Translation
• arabidopsis thaliana
• membrane proteins
• plasma membrane
• post-translational modification
• protein complexes
• roots

• UMR de Génétique Végétale, La Ferme du Moulon, Gif-sur-Yvette, France

  Hervé Thiellement, Michel Zivy, Catherine Damerval

• UMR de Chimie Biologique, Thiverval Grignon, France

  Valérie Méchin

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