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Plant Proteomics

Methods and Protocols

  • Hervé Thiellement
  • Michel Zivy
  • Catherine Damerval
  • Valérie Méchin

Part of the Methods in Molecular Biology book series (MIMB, volume 355)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Valérie Méchin, Catherine Damerval, Michel Zivy
    Pages 1-8
  3. Mireille Faurobert, Esther Pelpoir, Jamila Chaïb
    Pages 9-14
  4. Gérard Branlard, Emmanuelle Bancel
    Pages 15-25
  5. Julia Kehr, Martijn Rep
    Pages 27-35
  6. Christophe Plomion, Céline Lalanne
    Pages 37-41
  7. Klaas J. van Wijk, Jean-Benoit Peltier, Lisa Giacomelli
    Pages 43-48
  8. Holger Eubel, Joshua L. Heazlewood, A. Harvey Millar
    Pages 49-62
  9. Fernando González-Camacho, Francisco Javier Medina
    Pages 63-72
  10. Setsuko Komatsu
    Pages 73-77
  11. Bonnie S. Watson, Lloyd W. Sumner
    Pages 79-92
  12. Thierry Rabilloud, Sylvie Luche, Véronique Santoni, Mireille Chevallet
    Pages 111-119
  13. Walter Weiss, Angelika Görg
    Pages 121-143
  14. François Chevalier, Valérie Rofidal, Michel Rossignol
    Pages 145-156
  15. Matthew Keeler, Jessica Letarte, Emily Hattrup, Fatimah Hickman, Paul A. Haynes
    Pages 157-174
  16. Michel Zivy
    Pages 175-194
  17. Kåre Engkilde, Susanne Jacobsen, Ib Søndergaard
    Pages 195-210
  18. Setsuko Komatsu
    Pages 211-217
  19. Nicolas Sommerer, Delphine Centeno, Michel Rossignol
    Pages 219-234
  20. Wojciech Szponarski, Frédéric Delom, Nicolas Sommerer, Michel Rossignol, Rémy Gibrat
    Pages 267-278
  21. Olivier Langella, Michel Zivy, Johann Joets
    Pages 279-303
  22. Maria V. Turkina, Alexander V. Vener
    Pages 305-316
  23. Anne-Catherine Fitchette, Olivia Tran Dinh, Loïc Faye, Muriel Bardor
    Pages 317-342
  24. Jesco Heinemeyer, Dagmar Lewejohann, Hans-Peter Braun
    Pages 343-352
  25. Zhentian Lei, Ajith Anand, Kirankumar S. Mysore, Lloyd W. Sumner
    Pages 353-363
  26. Tanja Feilner, Birgit Kersten
    Pages 379-390
  27. Back Matter
    Pages 391-399

About this book

Introduction

The aim of Plant Proteomics: Methods and Protocols is to present up-- date methods and protocols used by recognized scientists in the world of plant proteomics. If this world was a very small one twenty-five years ago when the first papers were published, it has since experienced exponential growth, and in most countries around the world there are laboratories working on plant proteomics. Two-dimensional gel electrophoresis is still the basic method used, but it has been improved greatly with IPG in the first dimension (Chapter 13) and with new detection methods with fluorochromes (Chapters 14 and 15). Signi- cant progress has been achieved in protein extraction, which is particularly difficult with plant tissues containing phenols, proteases, and other secondary metabolites that interfere with proteins. Standard procedures have been op- mized (Chapters 1 and 2) for peculiar tissues (Chapters 3, 4, and 5) and cellular compartments (Chapters 6 to 10). These methods rely on improvements made in the solubilization of proteins from membranes (Chapters 11 and 12). Mass spectrometry was a revolution that permitted the high throughput identifi- tion of proteins separated by 2D gels (Chapters 19 and 20) but also from blue native 1D gels (Chapters 27 and 28) despite the fact that Edman sequencing can still be useful (Chapter 18). Associated with other techniques such as 2DLC or LC of intact proteins, mass spectrometry also permits the identification of polypeptides from complexes (Chapters 21 and 22).

Keywords

Microarray Polyacrylamid-Gelelektrophorese Proteomics Seed Translation arabidopsis thaliana membrane proteins plasma membrane post-translational modification protein complexes roots

Editors and affiliations

  • Hervé Thiellement
    • 1
  • Michel Zivy
    • 1
  • Catherine Damerval
    • 1
  • Valérie Méchin
    • 2
  1. 1.UMR de Génétique Végétale, La Ferme du MoulonGif-sur-YvetteFrance
  2. 2.UMR de Chimie BiologiqueThiverval GrignonFrance

Bibliographic information

  • DOI https://doi.org/10.1385/1597452270
  • Copyright Information Humana Press 2007
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-635-1
  • Online ISBN 978-1-59745-227-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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