Agrobacterium Protocols

  • Kan Wang

Part of the Methods in Molecular Biology book series (MIMB, volume 343)

Table of contents

  1. Front Matter
    Pages i-xxii

    1. Front Matter
      Pages 1-1
    2. Arlene A. Wise, Zhenying Liu, Andrew N. Binns
      Pages 3-14
    3. Toshihiko Komari, Yoshimitsu Takakura, Jun Ueki, Norio Kato, Yuji Ishida, Yukoh Hiei
      Pages 15-42
    4. Arlene A. Wise, Zhenying Liu, Andrew N. Binns
      Pages 43-54
    5. Arlene A. Wise, Zhenying Liu, Andrew N. Binns
      Pages 67-76
    6. Stanton B. Gelvin
      Pages 77-85
  3. Model Plants

    1. Front Matter
      Pages 85-85
    2. Viviane Cosson, Patricia Durand, Isabelle d'Erfurth, Adam Kondorosi, Pascal Ratet
      Pages 115-128
    3. Elane Wright, Richard A. Dixon, Zeng-Yu Wang
      Pages 129-136
    4. Cynthia Crane, Richard A. Dixon, Zeng-Yu Wang
      Pages 137-142
    5. Christopher G. Taylor, Beth Fuchs, Ray Collier, W. Kevin Lutke
      Pages 155-168
  4. Cereal Crops

    1. Front Matter
      Pages 169-169
    2. John Jacobsen, Ingrid Venables, Ming-Bo Wang, Peter Matthews, Michael Ayliffe, Frank Gubler
      Pages 171-184
    3. Bronwyn R. Frame, Tina Paque, Kan Wang
      Pages 185-200
    4. Karabi Datta, Swapan Kumar Datta
      Pages 201-212

About this book


Rapid changes and significant progress have been made in the use of Agrobacterium to genetically transform plants for both basic research purposes and agricultural development. In Agrobacterium Protocols, Second Edition, Volumes 1 and 2, a team of leading experts and veteran researchers describe in detail their best techniques for delivering DNA to plant cells and permanently altering their genomes. Volume 1 details the most updated techniques available for twenty-six plant species drawn from cereal crops, industrial plants, legume plants, and vegetable plants, and presents various methods for introducing DNA into three major model plant species, Arabidopsis thaliana, Medicago truncatula, and Nicotiana. The authors also outline the basic methods in Agrobacterium manipulation and strategies for vector construction, major components of plant transformation that are often neglected by many plant biologists. Volume 2 contains another thirty-three proven techniques for root plants, turf grasses, woody species, tropic plants, nuts and fruits, ornamental plants, and medicinal plants. Additional chapters provide methods for introducing DNA into non-plant species, such as bacteria, fungi, algae, and mammalian cells. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Agrobacterium Protocols, Second Edition, Volumes 1 and 2 offers plant biotechnologists a gold standard collection of Agrobacterium-mediated transformation techniques for state-of-the-art plant genetic engineering, functional genomic analysis, and crop improvement, and for inspiration in developing new methods for other related and non-related plants.


Agrobacterium tumefaciens Arabidopsis thaliana DNA Flora Zea mays L. algae bean fungi genes genetic engineering molecular biology pisum sativum l. virulence wheat

Editors and affiliations

  • Kan Wang
    • 1
  1. 1.Center for Plant Transformation, Plant Science Institute, and Department of AgronomyIowa State UniversityAmesUSA

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-536-1
  • Online ISBN 978-1-59745-130-7
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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