MicroRNA Protocols

1. In Vitro Precursor MicroRNA Processing Assays Using Drosophila Schneider-2 Cell Lysates

   • Akira Ishizuka, Kuniaki Saito, Mikiko C. Siomi, Haruhiko Siomi

   Pages 277-286

2. Downregulation of Human Cdc6 Protein Using a Lentivirus RNA Interference Expression Vector

   • Feng Luo, Jiing-Kuan Yee, Sheng-He Huang, Ling-Tao Wu, Ambrose Y. Jong

   Pages 287-293

3. Gene Silencing In Vitro and In Vivo Using Intronic MicroRNAs

   • Shi-Lung Lin, Shao-Yao Ying

   Pages 295-312

4. Isolation and Identification of Gene-Specific MicroRNAs

   • Shi-Lung Lin, Donald C. Chang, Shao-Yao Ying

   Pages 313-320

5. Transgene-Like Animal Models Using Intronic MicroRNAs

   • Shi-Lung Lin, Shin-Ju E. Chang, Shao-Yao Ying

   Pages 321-334

6. Evolution of MicroRNAs

   • Andrea Tanzer, Peter F. Stadler

   Pages 335-350

7. Perspectives

   • Shao-Yao Ying, Donald C. Chang, Joseph D. Miller, Shi-Lung Lin

   Pages 351-358

8. Back Matter

   Pages 359-365

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MicroRNA Protocols provides diverse, novel, and useful descriptions of miRNAs in several species, including plants, worms, flies, fish, chicks, mice, and humans. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the identification of miRNAs. This volume will stimulate the reader to explore diverse ways to understanding the mechanism in which miRNAs facilitate the molecular aspects of the biomedical research.

• Vivo
• evolution
• genes
• hybridization
• miRNAs
• tissue

• Department of Cell and Neurobiology, Keck School of Medicine, University of Southern California, Los Angeles, USA

  Shao-Yao Ying

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