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Clinical Applications of PCR

  • Y. M. Dennis Lo
  • Rossa W. K. Chiu
  • K. C. Allen Chan

Part of the Methods in Molecular Biology™ book series (MIMB, volume 336)

Table of contents

  1. Front Matter
    Pages i-x
  2. Y. M. Dennis Lo, K. C. Allen Chan
    Pages 1-10
  3. Y. M. Dennis Lo, K. C. Allen Chan
    Pages 11-18
  4. Robert J. Pryor, Carl T. Wittwer
    Pages 19-32
  5. T. Vauvert Hviid
    Pages 45-58
  6. Bernhard Zimmermann, Lisa Levett, Wolfgang Holzgreve, Sinuhe Hahn
    Pages 83-100
  7. Rossa W. K. Chiu, Y. M. Dennis Lo
    Pages 101-109
  8. Nancy B. Y. Tsui, Ng Enders K. O., Y. M. Dennis Lo
    Pages 123-134
  9. Lee-JunC. Wong, Bryan R. Cobb, Tian-Jian Chen
    Pages 135-143
  10. Anatoly V. Lichtenstein, Hovsep S. Melkonyan, L. David Tomei, Samuil R. Umansky
    Pages 145-154
  11. Enders K. O. Ng, Y. M. Dennis Lo
    Pages 163-175
  12. Stephen S. C. Chim, Rossa W. K. Chiu, Y. M. Dennis Lo
    Pages 177-194
  13. Back Matter
    Pages 195-200

About this book

Introduction

In this updated second edition of the much praised Clinical Applications of PCR, leading researchers apply molecular diagnostics to the many recent advances that have occurred in polymerase chain reaction (PCR)-based technologies. Highlights include real-time PCR, which allows the technique to be performed in a quantitative manner with improved sensitivity, robustness, and resilience to carryover contamination, mass spectrometric analysis of nucleic acids, and circulating cell-free nucleic acids in plasma. The authors apply these innovations to a broad spectrum of applications, including gene expression, methylation, trace molecule, gene dosage, and single cell analysis. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Clinical Applications of PCR, Second Edition, will help today's laboratorians and researchers working in molecular diagnostics harness the power of these techniques with unprecedented sensitivity and ease.

Keywords

DNA Laboratory Mutation PCR gene expression

Editors and affiliations

  • Y. M. Dennis Lo
    • 1
  • Rossa W. K. Chiu
    • 1
  • K. C. Allen Chan
    • 1
  1. 1.Department of Chemical PathologyThe Chinese University of Hong KongHong Kong SARChina

Bibliographic information

  • DOI https://doi.org/10.1385/159745074X
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-348-0
  • Online ISBN 978-1-59745-074-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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