Clinical Applications of PCR

1. Front Matter

   Pages i-x

   PDF

2. Introduction to the Polymerase Chain Reaction

   • Y. M. Dennis Lo, K. C. Allen Chan

   Pages 1-10

3. Setting Up a Polymerase Chain Reaction Laboratory

   • Y. M. Dennis Lo, K. C. Allen Chan

   Pages 11-18

4. Real-Time Polymerase Chain Reaction and Melting Curve Analysis

   • Robert J. Pryor, Carl T. Wittwer

   Pages 19-32

5. Qualitative and Quantitative Polymerase Chain Reaction-Based Methods for DNA Methylation Analyses

   • Ivy H. N. Wong

   Pages 33-43

6. In-Cell Polymerase Chain Reaction

   • T. Vauvert Hviid

   Pages 45-58

7. Qualitative and Quantitative DNA and RNA Analysis by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

   • Chunming Ding

   Pages 59-71

8. Analysis of Polymerase Chain Reaction Products by Denaturing High-Performance Liquid Chromatography

   • Ching-Wan Lam

   Pages 73-82

9. Use of Real-Time Polymerase Chain Reaction for the Detection of Fetal Aneuploidies

   • Bernhard Zimmermann, Lisa Levett, Wolfgang Holzgreve, Sinuhe Hahn

   Pages 83-100

10. Noninvasive Prenatal Diagnosis by Analysis of Fetal DNA in Maternal Plasma

    • Rossa W. K. Chiu, Y. M. Dennis Lo

    Pages 101-109

11. Clinical Applications of Plasma Epstein-Barr Virus DNA Analysis and Protocols for the Quantitative Analysis of the Size of Circulating Epstein-Barr Virus DNA

    • K. C. Allen Chan, Y. M. Dennis Lo

    Pages 111-121

12. Molecular Analysis of Circulating RNA in Plasma

    • Nancy B. Y. Tsui, Ng Enders K. O., Y. M. Dennis Lo

    Pages 123-134

13. Molecular Analysis of Mitochondrial DNA Point Mutations by Polymerase Chain Reaction

    • Lee-JunC. Wong, Bryan R. Cobb, Tian-Jian Chen

    Pages 135-143

14. Novel Applications of Polymerase Chain Reaction to Urinary Nucleic Acid Analysis

    • Anatoly V. Lichtenstein, Hovsep S. Melkonyan, L. David Tomei, Samuil R. Umansky

    Pages 145-154

15. Detection and Quantitation of Circulating Plasmodium falciparum DNA by Polymerase Chain Reaction

    • Shira Gal, James S. Wainscoat

    Pages 155-162

16. Molecular Diagnosis of Severe Acute Respiratory Syndrome

    • Enders K. O. Ng, Y. M. Dennis Lo

    Pages 163-175

17. Genomic Sequencing of the Severe Acute Respiratory Syndrome-Coronavirus

    • Stephen S. C. Chim, Rossa W. K. Chiu, Y. M. Dennis Lo

    Pages 177-194

18. Back Matter

    Pages 195-200

    PDF

In this updated second edition, leading researchers apply molecular diagnostics to the many recent advances that have occurred in polymerase chain reaction( PCR)-based technologies. Highlights include real-time PCR, which allows the technique to be performed in a quantitative manner with improved sensitivity, robustness, and resilience to carryover contamination, mass spectrometric analysis of nucleic acids, and circulating cell-free nucleic acids in plasma. The authors apply these innovations to a broad spectrum of applications, including gene expression, methylation, trace molecule, gene dosage, and single cell analysis.

• DNA
• Laboratory
• Mutation
• PCR
• gene expression

From the reviews:

"...will be an important laboratory resource to those using PCR in the diagnosis of human diseases and should be of great interest to both molecular and clinical pathologists." -Archives of Pathology and Laboratory Medicine

"Each chapter is clearly written and provides enough background material to explain the significance and application of the procedure. The protocols include sufficient detail to enable the reader to develop the test. This manual will be an important laboratory resource to those using PCR in the diagnosis of human diseases and should be of great interest to both molecular and clinical pathologists."-Archives of Pathology and Laboratory Medicine

"...a unique document that will be welcome in the clinical laboratory as well as the research field." -Doody's Health Sciences Book Review Journal

"This book is pitched at both pathologists and microbiologists who use PCR in a diagnostic setting. It provides a user-friendly and relatively brief theoretical background to conventional and real-time PCR … . This book should appeal to professional laboratory scientists and students … as well as to more experienced individuals who want to use or extend specific applications. This book would be a useful purchase as a reference source and technical manual for diagnostic pathology and microbiology laboratories." (David Hampson, SGM- Society for General Microbiology, 2007)

• Department of Chemical Pathology, The Chinese University of Hong Kong, Hong Kong SAR, China

  Y. M. Dennis Lo, Rossa W. K. Chiu, K. C. Allen Chan

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