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Microfluidic Techniques

Reviews and Protocols

  • Shelley D. Minteer

Part of the Methods In Molecular Biology™ book series (MIMB, volume 321)

Table of contents

  1. Front Matter
    Pages i-x
  2. Microfabrication Methods

    1. Front Matter
      Pages 3-3
    2. Shelley D. Minteer, Christine M. Moore
      Pages 1-2
    3. Rabih Zaouk, Benjamin Y. Park, Marc J. Madou
      Pages 5-15
    4. Rabih Zaouk, Benjamin Y. Park, Marc J. Madou
      Pages 17-21
    5. Benjamin Y. Park, Rabih Zaouk, Marc J. Madou
      Pages 23-26
  3. Biological Applications

    1. Front Matter
      Pages 44-44
    2. Gloria Thomas, Emad M. El-Giar, Laurie E. Locascio, Michael J. Tarlov
      Pages 83-95
    3. Kalee D. Spitzack, Victor M. Ugaz
      Pages 97-129
    4. Maria C. Carles, Nikolaus J. Sucher
      Pages 131-140
    5. Xiaojun Zhao, David T. Pierce, Yanfu Huan
      Pages 141-155
    6. Shelley D. Minteer, Christine M. Moore
      Pages 157-166
    7. Anne M. Taylor, Seog Woo Rhee, Noo Li Jeon
      Pages 167-177
    8. Yaokuang Chung, Xiaoyue Zhu, Wei Gu, Gary D. Smith, Shuichi Takayama
      Pages 227-244
  4. Back Matter
    Pages 245-147

About this book

Introduction

Over the last ten years, miniaturization has dramatically shrunk the size of both cellular telephones and chemical instrumentation, and now also holds the promise of developing portable, inexpensive, and disposable analytical systems on a microchip. In Microfluidic Techniques: Reviews and Protocols, hands-on researchers review the principles behind successful miniaturization and describe the key techniques for miniaturizing large-scale biochemical and bioanalytical methods for microchip analysis. The authors cover not only the most popular methods for the fabrication of microchips (photolithography, laser ablation, and soft lithography), but also microfluidic techniques for such bioanalytical assays and bioprocesses as DNA analysis, polymerase chain reaction (PCR), immunoassays, and cell reactors. Highlights include PCR on a microchip, microscale cell culturing, and the study of cellular processes on a microchip. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and informative, Microfluidic Techniques: Reviews and Protocols synthesizes the chemistry, biology, and engineering underlying microfluidic devices so that molecular biologists, as well as analytical and clinical chemists, can advance easily from macroscale to microscale biochemical and bioanalytical techniques.

Keywords

DNA PCR cellular processes ethanol migration

Editors and affiliations

  • Shelley D. Minteer
    • 1
  1. 1.Department of ChemistrySaint Louis UniversitySt. Louis

Bibliographic information

  • DOI https://doi.org/10.1385/1592599974
  • Copyright Information Humana Press 2006
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-517-0
  • Online ISBN 978-1-59259-997-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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