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  • © 2005

Mast Cells

Methods and Protocols

Part of the book series: Methods in Molecular Biology (MIMB, volume 315)

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  • ISBN: 978-1-59259-967-7
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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xvii
  2. Overview of Mast Cell Biology

    1. Front Matter

      Pages 1-1
    2. The Human Mast Cell

      • Guha Krishnaswamy, Omar Ajitawi, David S. Chi
      Pages 13-34
    3. Mast Cells in Allergy and Autoimmunity

      • Gregory D. Gregory, Melissa A. Brown
      Pages 35-50
  3. Identification of Mast Cells in Culture and in Tissue

    1. Front Matter

      Pages 51-51
    2. Analysis of MCT and MCTC Mast Cells in Tissue

      • Lawrence B. Schwartz
      Pages 53-62
    3. Mast Cell Ultrastructure and Staining in Tissue

      • Shruti A. Shukla, Ranjitha Veerappan, Judy S. Whittimore, Lou Ellen Miller, George A. Youngberg
      Pages 63-76
    4. Expression of Cell Surface Antigens on Mast Cells

      • Alexander W. Hauswirth, Stefan Florian, Gerit-Holger Schernthaner, Maria-Theresa Krauth, Karoline Sonneck, Wolfgang R. Sperr et al.
      Pages 77-90
    5. Identification of Mast Cells in the Cellular Response to Myocardial Infarction

      • Nikolaos G. Frangogiannis, Mark L. Entman
      Pages 91-101
  4. Development of Mast Cells In Vitro

    1. Front Matter

      Pages 103-103
    2. Isolation, Culture, and Characterization of Intestinal Mast Cells

      • Gernot Sellge, Stephan C. Bischoff
      Pages 123-138
  5. Mast Cell Signaling and Gene Expression

    1. Front Matter

      Pages 139-139
    2. Activation of Nuclear Factor-κB

      • Chuanfu Li, Jim Kelley, Tuanzhu Ha
      Pages 141-150
    3. Analysis of Mitogen-Activated Protein Kinase Activation

      • Stephen C. Armstrong
      Pages 151-163
    4. Microarray and Gene-Clustering Analysis

      • Tsung-Hsien Tsai, Denise M. Milhorn, Shau-Ku Huang
      Pages 165-174
    5. Techniques to Study FcεRI Signaling

      • Yuko Kawakami, Jiro Kitaura, Toshiaki Kawakami
      Pages 175-189
  6. Mast Cell Expression and Release of Inflammatory Mediators of Inflammatory Mediators

    1. Front Matter

      Pages 191-191

About this book

The mast cell, long implicated in causing allergic reactions, may also be involved in many other disease processes, including cancer, heart disease, parasitic disease, atherosclerosis, asthma, and arthritis. In Mast Cells: Methods and Protocols, hands-on experts describe in detail their best techniques for the isolation, culture, and study of both activation and signaling in human mast cells. These readily reproducible methods take advantage of the latest advances in molecular biology, technology, and information science. The techniques provide a sound base of methodology for mast cell research and include methods for the identification of mast cells, the development of mast cells in vitro, the study of mast cell signaling and gene expression, and the measurement of mast cell expression of inflammatory mediators. Additional chapters cover methods for studying mast cell interactions with other cell types (endothelial cells, fibroblasts, and B cells), the roles of mast cells in host defense, and mast cell apoptosis. A survey of mast cell biology offers insight into its history and the implications for adaptive immunity. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Mast Cells: Methods and Protocols provides mast cell researchers with reproducible accounts of basic and advanced molecular and cellular techniques used in studying this fascinating, multifunctional cell.

Keywords

  • Antigen
  • DNA
  • RNA
  • biology
  • cell biology
  • cytokine
  • development
  • gene expression
  • genes
  • microarray
  • molecular biology
  • protein
  • regulation
  • reproducible techniques
  • tissue

Reviews

From the reviews:

"This volume provides a set of protocols for investigators working with mast cells. Each of the chapters is accompanied by a short bibliography. … The information will be of value to advanced students and laboratory investigators. … Each of the substantive chapters contains detailed protocols and a short set of primary references. … there is sufficient detail to allow direct adaptation in the laboratory. … the volume contains useful material for investigators working in this area." (Eugene A Davidson, Doody’s Book Review Service, March, 2006)

Editors and Affiliations

  • Quillen College of Medicine, James H. Quillen Veterans Affairs Medical Center, East Tennessee State University, Johnson City

    Guha Krishnaswamy, David S. Chi

Bibliographic Information

  • Book Title: Mast Cells

  • Book Subtitle: Methods and Protocols

  • Editors: Guha Krishnaswamy, David S. Chi

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592599672

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2005

  • Hardcover ISBN: 978-1-58829-374-9

  • Softcover ISBN: 978-1-61737-527-9

  • eBook ISBN: 978-1-59259-967-7

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVIII, 439

  • Number of Illustrations: 115 b/w illustrations

  • Topics: Immunology

  • Industry Sectors: Biotechnology, Health & Hospitals, Health & Hospitals, Pharma

Buying options

eBook USD 149.00
Price excludes VAT (USA)
  • ISBN: 978-1-59259-967-7
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 199.99
Price excludes VAT (USA)
Hardcover Book USD 199.99
Price excludes VAT (USA)