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  • © 2006

Plant Cell Culture Protocols

Part of the book series: Methods in Molecular Biology (MIMB, volume 318)

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  • ISBN: 978-1-59259-959-2
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Table of contents (30 protocols)

  1. Applications for Plant Protoplasts

    1. Front Matter

      Pages 199-199
    2. Isolation, Culture, and Plant Regeneration From Leaf Protoplasts of Passiflora

      • Michael R. Davey, Paul Anthony, J. Brian Power, Kenneth C. Lowe
      Pages 201-210
    3. Isolation, Culture, and Plant Regeneration From Echinacea purpurea Protoplasts

      • Zeng-guang Pan, Chun-zhao Liu, Susan J. Murch, Praveen K. Saxena
      Pages 211-217
    4. Production of Cybrids in Brassicaceae Species

      • Maksym Vasylenko, Olga Ovcharenko, Yuri Gleba, Nikolay Kuchuk
      Pages 219-232
    5. Guard Cell Protoplasts

      • Gary Tallman
      Pages 233-252
    6. Production of Interspecific Hybrid Plants in Primula

      • Juntaro Kato, Masahiro Mii
      Pages 253-262
  2. Protocols for Genomic Manipulation

    1. Front Matter

      Pages 263-263
    2. Transformation of Wheat Via Particle Bombardment

      • Indra K. Vasil, Vimla Vasil
      Pages 273-283
    3. Chloroplast Transformation

      • Xiao-Mei Lu, Wei-Bo Yin, Zan-Min Hu
      Pages 285-303
    4. The Biochemical Basis for the Resistance to Aluminum and Their Potential as Selection Markers

      • César De los Santos-Briones, Teresa Hernández-Sotomayor
      Pages 305-314
  3. Accumulation of Metabolites in Plant Cells

    1. Front Matter

      Pages 325-325
    2. Isolation and Purification of Ribosome-Inactivating Proteins

      • Sang-Wook Park, Balakrishnan Prithiviraj, Ramarao Vepachedu, Jorge M. Vivanco
      Pages 335-347
    3. Catharanthus roseus Shoot Cultures for the Production of Monoterpenoid Indole Alkaloids

      • Elizabeta Hernández-Domínguez, Freddy Campos-Tamayo, Mildred Carrillo-Pech, Felipe Vázquez-Flota
      Pages 349-355
    4. Methods for Regeneration and Transformation in Eschscholzia californica

      • Benjamin P. MacLeod, Peter J. Facchini
      Pages 357-368
  4. Back Matter

    Pages 369-394

About this book

Following in the footsteps of its much-acclaimed first edition, Plant Cell Culture Protocols, Second Edition has been expanded and revised to include the most up-to-date and frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Two new appendices have also been added to explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, as well as to list more than 100 very useful internet sites.
Comprehensive and highly practical, Plant Cell Culture Protocols, Second Edition offers today's plant biotechnologists indispensable state-of-the-art techniques for carrying out plant research, for developing new transgenic plants, and for today's many and diverse commercial applications.

Keywords

  • Agrobacterium tumefaciens
  • Brassicaceae
  • Chloroplast
  • Embryo
  • Flora
  • Pathogen
  • Protein
  • biotechnology
  • cell division
  • cell expansion
  • cryopreservation
  • wheat

Reviews

From Reviews of the First Edition...

"...an excellent addition to the library of researchers who wish to extend their collection of basic cell culture techniques" -Quarterly Review of Biology

"...represents a valuable resource of information on in vitro culture methods in plants, which will find extensive application on the laboratory bench." -Plant Science

"...thoroughly recommend[ed]..." -Plant Growth Regulation

"...undoubtedly represents one of the highest quality manuals on this topic published within the last ten years." -Biologia Plantarum

Editors and Affiliations

  • Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigacion Cientifica de Yucatán, Yucatán, México

    Victor M. Loyola-Vargas, Felipe Vázquez-Flota

Bibliographic Information

  • Book Title: Plant Cell Culture Protocols

  • Editors: Victor M. Loyola-Vargas, Felipe Vázquez-Flota

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592599591

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Hardcover ISBN: 978-1-58829-547-7

  • Softcover ISBN: 978-1-61737-634-4

  • eBook ISBN: 978-1-59259-959-2

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 394

  • Number of Illustrations: 74 b/w illustrations, 3 illustrations in colour

  • Topics: Cell Biology, Plant Science, Plant Physiology

  • Industry Sectors: Biotechnology, Health & Hospitals, Pharma

Buying options

eBook USD 139.00
Price excludes VAT (USA)
  • ISBN: 978-1-59259-959-2
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 179.00
Price excludes VAT (USA)
Hardcover Book USD 219.99
Price excludes VAT (USA)