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Plant Cell Culture Protocols

  • Book
  • © 2006

Overview

Editors:
  1. Victor M. Loyola-Vargas

    1. Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigacion Cientifica de Yucatán, Yucatán, México

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  2. Felipe Vázquez-Flota

    1. Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigacion Cientifica de Yucatán, Yucatán, México

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Part of the book series: Methods in Molecular Biology (MIMB, volume 318)

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Table of contents (30 protocols)

  1. Applications for Plant Protoplasts

    1. Front Matter

      Pages 199-199
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    2. Isolation, Culture, and Plant Regeneration From Leaf Protoplasts of Passiflora

      • Michael R. Davey, Paul Anthony, J. Brian Power, Kenneth C. Lowe
      Pages 201-210

    3. Isolation, Culture, and Plant Regeneration From Echinacea purpurea Protoplasts

      • Zeng-guang Pan, Chun-zhao Liu, Susan J. Murch, Praveen K. Saxena
      Pages 211-217

    4. Production of Cybrids in Brassicaceae Species

      • Maksym Vasylenko, Olga Ovcharenko, Yuri Gleba, Nikolay Kuchuk
      Pages 219-232

    5. Guard Cell Protoplasts

      • Gary Tallman
      Pages 233-252

    6. Production of Interspecific Hybrid Plants in Primula

      • Juntaro Kato, Masahiro Mii
      Pages 253-262

  2. Protocols for Genomic Manipulation

    1. Front Matter

      Pages 263-263
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    2. Agrobacterium-Mediated Transformation of Petunia Leaf Discs

      • Ingrid M. van der Meer
      Pages 265-272

    3. Transformation of Wheat Via Particle Bombardment

      • Indra K. Vasil, Vimla Vasil
      Pages 273-283

    4. Chloroplast Transformation

      • Xiao-Mei Lu, Wei-Bo Yin, Zan-Min Hu
      Pages 285-303

    5. The Biochemical Basis for the Resistance to Aluminum and Their Potential as Selection Markers

      • César De los Santos-Briones, Teresa Hernández-Sotomayor
      Pages 305-314

    6. Transformation of Maize Via Agrobacterium tumefaciens Using a Binary Co-Integrate Vector System

      • Zuo-yu Zhao, Jerry Ranch
      Pages 315-323

  3. Accumulation of Metabolites in Plant Cells

    1. Front Matter

      Pages 325-325
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    2. Capsaicin Accumulation in Capsicum spp. Suspension Cultures

      • Neftalí Ochoa-Alejo
      Pages 327-334

    3. Isolation and Purification of Ribosome-Inactivating Proteins

      • Sang-Wook Park, Balakrishnan Prithiviraj, Ramarao Vepachedu, Jorge M. Vivanco
      Pages 335-347

    4. Catharanthus roseus Shoot Cultures for the Production of Monoterpenoid Indole Alkaloids

      • Elizabeta Hernández-Domínguez, Freddy Campos-Tamayo, Mildred Carrillo-Pech, Felipe Vázquez-Flota
      Pages 349-355

    5. Methods for Regeneration and Transformation in Eschscholzia californica

      • Benjamin P. MacLeod, Peter J. Facchini
      Pages 357-368

  4. Back Matter

    Pages 369-394
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Keywords

About this book

A comprehensive state-of-the-art collection of the most frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. Two new appendices explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, and list more than 100 very useful internet sites.

Reviews

From Reviews of the First Edition...

"...an excellent addition to the library of researchers who wish to extend their collection of basic cell culture techniques" -Quarterly Review of Biology

"...represents a valuable resource of information on in vitro culture methods in plants, which will find extensive application on the laboratory bench." -Plant Science

"...thoroughly recommend[ed]..." -Plant Growth Regulation

"...undoubtedly represents one of the highest quality manuals on this topic published within the last ten years." -Biologia Plantarum

Editors and Affiliations

  • Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigacion Cientifica de Yucatán, Yucatán, México

    Victor M. Loyola-Vargas, Felipe Vázquez-Flota

Bibliographic Information

  • Book Title: Plant Cell Culture Protocols

  • Editors: Victor M. Loyola-Vargas, Felipe Vázquez-Flota

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592599591

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Hardcover ISBN: 978-1-58829-547-7Published: 01 November 2005

  • Softcover ISBN: 978-1-61737-634-4Published: 09 December 2010

  • eBook ISBN: 978-1-59259-959-2Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 394

  • Number of Illustrations: 74 b/w illustrations, 3 illustrations in colour

  • Topics: Cell Biology, Plant Sciences, Plant Physiology

  • Industry Sectors: Biotechnology, Pharma

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