Plant Cell Culture Protocols

1. Front Matter

   Pages i-xv

   PDF

2. Introduction

   1. Front Matter

      Pages 1-1

      PDF

   2. An Introduction to Plant Cell Culture

      • Victor M. Loyola-Vargas, Felipe Vázquez-Flota

      Pages 3-8

   3. History of Plant Tissue Culture

      • Trevor A. Thorpe

      Pages 9-32

3. Cell Culture and Plant Regeneration: The Fundamentals

   1. Front Matter

      Pages 33-33

      PDF

   2. Pathogen and Biological Contamination Management

      • Alan C. Cassells, Barbara M. Doyle

      Pages 35-50

   3. Growth Measurements

      • Gregorio Godoy-Hernández, Felipe A. Vázquez-Flota

      Pages 51-58

   4. Callus and Suspension Culture Induction, Maintenance, and Characterization

      • Alejandrina Robledo-Paz, María Nélida Vázquez-Sánchez, Rosa María Adame-Alvarez, Alba Estela Jofre-Garfias

      Pages 59-70

   5. Measurement of Cell Viability in In Vitro Cultures

      • Lizbeth A. Castro-Concha, Rosa María Escobedo, María Lourdes de Miranda-Ham

      Pages 71-76

   6. Cryopreservation of Embryogenic Cell Suspensions by Encapsulation-Vitrification

      • Qiaochun Wang, Avihai Perl

      Pages 77-86

   7. Somatic Embryogenesis in Picea Suspension Cultures

      • Claudio Stasolla

      Pages 87-99

   8. Indirect Somatic Embryogenesis in Cassava for Genetic Modification Purposes

      • Krit Raemakers, Isolde Pereira, Herma Koehorst van Putten, Richard Visser

      Pages 101-109

   9. Direct Somatic Embryogenesis in Coffea canephora

      • Francisco Quiroz-Figueroa, Miriam Monforte-González, Rosa M. Galaz-Ávalos, Victor M. Loyola-Vargas

      Pages 111-117

4. PLANT PROPAGATION IN VITRO

   1. Front Matter

      Pages 119-119

      PDF

   2. A New Temporary Immersion Bioreactor System for Micropropagation

      • Manuel L. Robert, José Luis Herrera-Herrera, Gastón Herrera-Herrera, Miguel Ángel Herrera-Alamillo, Pedro Fuentes-Carrillo

      Pages 121-129

   3. Protocol to Achieve Photoautotrophic Coconut Plants Cultured In Vitro With Improved Performance Ex Vitro

      • Gabriela Fuentes, Carlos Talavera, Yves Desjardins, Jorge M. Santamaría

      Pages 131-144

   4. Use of Statistics in Plant Biotechnology

      • Michael E. Compton

      Pages 145-163

   5. An Efficient Method for the Micropropagation of Agave Species

      • Manuel L. Robert, José Luis Herrera-Herrera, Eduardo Castillo, Gabriel Ojeda, Miguel Angel Herrera-Alamillo

      Pages 165-178

   6. Micropropagation of Endangered Plant Species

      • Zhihua Liao, Min Chen, Xiaofen Sun, Kexuan Tang

      Pages 179-185

   7. Clonal Propagation of Softwoods

      • Trevor A. Thorpe, Indra S. Harry, Edward C. Yeung

      Pages 187-197

A comprehensive state-of-the-art collection of the most frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. Two new appendices explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, and list more than 100 very useful internet sites.

• Agrobacterium tumefaciens
• Brassicaceae
• Chloroplast
• Embryo
• Flora
• Pathogen
• Protein
• biotechnology
• cell division
• cell expansion
• cryopreservation
• wheat

From Reviews of the First Edition...

"...an excellent addition to the library of researchers who wish to extend their collection of basic cell culture techniques" -Quarterly Review of Biology

"...represents a valuable resource of information on in vitro culture methods in plants, which will find extensive application on the laboratory bench." -Plant Science

"...thoroughly recommend[ed]..." -Plant Growth Regulation

"...undoubtedly represents one of the highest quality manuals on this topic published within the last ten years." -Biologia Plantarum

• Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigacion Cientifica de Yucatán, Yucatán, México

  Victor M. Loyola-Vargas, Felipe Vázquez-Flota

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