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  • © 2006

Plant Cell Culture Protocols

Part of the book series: Methods in Molecular Biology (MIMB, volume 318)

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eBook USD 139.00
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  • ISBN: 978-1-59259-959-2
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Softcover Book USD 179.00
Price excludes VAT (USA)
Hardcover Book USD 219.99
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Table of contents (30 protocols)

  1. Front Matter

    Pages i-xv
  2. Introduction

    1. Front Matter

      Pages 1-1
    2. An Introduction to Plant Cell Culture

      • Victor M. Loyola-Vargas, Felipe Vázquez-Flota
      Pages 3-8
    3. History of Plant Tissue Culture

      • Trevor A. Thorpe
      Pages 9-32
  3. Cell Culture and Plant Regeneration: The Fundamentals

    1. Front Matter

      Pages 33-33
    2. Pathogen and Biological Contamination Management

      • Alan C. Cassells, Barbara M. Doyle
      Pages 35-50
    3. Growth Measurements

      • Gregorio Godoy-Hernández, Felipe A. Vázquez-Flota
      Pages 51-58
    4. Callus and Suspension Culture Induction, Maintenance, and Characterization

      • Alejandrina Robledo-Paz, María Nélida Vázquez-Sánchez, Rosa María Adame-Alvarez, Alba Estela Jofre-Garfias
      Pages 59-70
    5. Measurement of Cell Viability in In Vitro Cultures

      • Lizbeth A. Castro-Concha, Rosa María Escobedo, María Lourdes de Miranda-Ham
      Pages 71-76
    6. Indirect Somatic Embryogenesis in Cassava for Genetic Modification Purposes

      • Krit Raemakers, Isolde Pereira, Herma Koehorst van Putten, Richard Visser
      Pages 101-109
    7. Direct Somatic Embryogenesis in Coffea canephora

      • Francisco Quiroz-Figueroa, Miriam Monforte-González, Rosa M. Galaz-Ávalos, Victor M. Loyola-Vargas
      Pages 111-117
  4. PLANT PROPAGATION IN VITRO

    1. Front Matter

      Pages 119-119
    2. A New Temporary Immersion Bioreactor System for Micropropagation

      • Manuel L. Robert, José Luis Herrera-Herrera, Gastón Herrera-Herrera, Miguel Ángel Herrera-Alamillo, Pedro Fuentes-Carrillo
      Pages 121-129
    3. Protocol to Achieve Photoautotrophic Coconut Plants Cultured In Vitro With Improved Performance Ex Vitro

      • Gabriela Fuentes, Carlos Talavera, Yves Desjardins, Jorge M. Santamaría
      Pages 131-144
    4. Use of Statistics in Plant Biotechnology

      • Michael E. Compton
      Pages 145-163
    5. An Efficient Method for the Micropropagation of Agave Species

      • Manuel L. Robert, José Luis Herrera-Herrera, Eduardo Castillo, Gabriel Ojeda, Miguel Angel Herrera-Alamillo
      Pages 165-178
    6. Micropropagation of Endangered Plant Species

      • Zhihua Liao, Min Chen, Xiaofen Sun, Kexuan Tang
      Pages 179-185
    7. Clonal Propagation of Softwoods

      • Trevor A. Thorpe, Indra S. Harry, Edward C. Yeung
      Pages 187-197

About this book

Following in the footsteps of its much-acclaimed first edition, Plant Cell Culture Protocols, Second Edition has been expanded and revised to include the most up-to-date and frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Two new appendices have also been added to explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, as well as to list more than 100 very useful internet sites.
Comprehensive and highly practical, Plant Cell Culture Protocols, Second Edition offers today's plant biotechnologists indispensable state-of-the-art techniques for carrying out plant research, for developing new transgenic plants, and for today's many and diverse commercial applications.

Keywords

  • Agrobacterium tumefaciens
  • Brassicaceae
  • Chloroplast
  • Embryo
  • Flora
  • Pathogen
  • Protein
  • biotechnology
  • cell division
  • cell expansion
  • cryopreservation
  • wheat

Reviews

From Reviews of the First Edition...

"...an excellent addition to the library of researchers who wish to extend their collection of basic cell culture techniques" -Quarterly Review of Biology

"...represents a valuable resource of information on in vitro culture methods in plants, which will find extensive application on the laboratory bench." -Plant Science

"...thoroughly recommend[ed]..." -Plant Growth Regulation

"...undoubtedly represents one of the highest quality manuals on this topic published within the last ten years." -Biologia Plantarum

Editors and Affiliations

  • Unidad de Bioquímica y Biología Molecular de Plantas, Centro de Investigacion Cientifica de Yucatán, Yucatán, México

    Victor M. Loyola-Vargas, Felipe Vázquez-Flota

Bibliographic Information

  • Book Title: Plant Cell Culture Protocols

  • Editors: Victor M. Loyola-Vargas, Felipe Vázquez-Flota

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592599591

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2006

  • Hardcover ISBN: 978-1-58829-547-7

  • Softcover ISBN: 978-1-61737-634-4

  • eBook ISBN: 978-1-59259-959-2

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XVI, 394

  • Number of Illustrations: 74 b/w illustrations, 3 illustrations in colour

  • Topics: Cell Biology, Plant Science, Plant Physiology

  • Industry Sectors: Biotechnology, Health & Hospitals, Pharma

Buying options

eBook USD 139.00
Price excludes VAT (USA)
  • ISBN: 978-1-59259-959-2
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 179.00
Price excludes VAT (USA)
Hardcover Book USD 219.99
Price excludes VAT (USA)