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Interferon Methods and Protocols

  • Book
  • © 2005

Overview

Editors:
  1. Daniel J. J. Carr

    1. Department of Ophthalmology, The University of Oklahoma Health and Sciences Center, Oklahoma City

    View editor publications

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Part of the book series: Methods in Molecular Medicine (MIMM, volume 116)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Biological and Clinical Basis for Molecular Studies of Interferons

    • Katie R. Pang, Jashin J. Wu, David B. Huang, Stephen K. Tyring, Samuel Baron
    Pages 1-23

  3. Interferon Research

    • Myriam S. Kunzi, Paula M. Pitha
    Pages 25-35

  4. Virus Infection and the Interferon Response

    • Marcus J. Korth, John C. Kash, Jeffrey C. Furlong, Michael G. Katze
    Pages 37-55

  5. Genomic DNA Affinity Chromatography

    • Jyothi Kumaran, Eleanor N. Fish
    Pages 57-67

  6. Protein Engineering of Interferon Alphas

    • Renqiu Hu, Ke-jian Lei, Joseph Bekisz, Kathryn C. Zoon
    Pages 69-80

  7. Assays for the Interferon-Induced Enzyme 2′,5′ Oligoadenylate Synthetases

    • Saumendra N. Sarkar, Mitali Pandey, Ganes C. Sen
    Pages 81-101

  8. A Convenient and Sensitive Fluorescence Resonance Energy Transfer Assay for RNase L and 2′,5′ Oligoadenylates

    • Chandar S. Thakur, Zan Xu, Zhengfu Wang, Zachary Novince, Robert H. Silverman
    Pages 103-113

  9. Regulation of Murine Interferon Regulatory Factor Gene Expression in the Central Nervous System Determined by Multiprobe RNase Protection Assay

    • Shalina S. Ousman, Iain L. Campbell
    Pages 115-134

  10. Mitogen-Activated Protein Kinase Pathways in Interferon Signaling

    • Antonella Sassano, Amit Verma, Leonidas C. Platanias
    Pages 135-150

  11. Development of an Interferon-Based Cancer Vaccine Protocol

    • W. Robert Fleischmann Jr., Tzu G. Wu
    Pages 151-166

  12. Type I Interferons as Regulators of the Differentiation/Activation of Human Dendritic Cells

    • Stefano M. Santini, Caterina Lapenta, Filippo Belardelli
    Pages 167-181

  13. Flow Cytometric Techniques for Studying Plasmacytoid Dendritic Cells in Mixed Populations

    • Stacey L. Olshalsky, Patricia Fitzgerald-Bocarsly
    Pages 183-194

  14. Analysis of Anti-Interferon Properties of the Herpes Simplex Virus Type I ICP0 Protein

    • Karen Mossman
    Pages 195-205

  15. Interferon Subtype Gene Therapy for Regulating Cytomegalovirus Disease

    • Cassandra M. James, Emmalene J. Bartlett, Josephine P. Mansfield, Vanessa S. Cull
    Pages 207-219

  16. Transfection of Müller Cells With Type I Interferon Transgenes

    • Benitta John-Philip, Daniel J. J. Carr
    Pages 221-232

  17. Back Matter

    Pages 233-236
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Keywords

About this book

A compendium of optimized methods to measure type I interferon efficacy as an antiproliferative or an antiviral agent. These cutting-edge techniques range from the simple to the highly complex and serve to illuminate the signaling cascades and the activation of enzymatic pathways prompted by interferon. The protocols follow the successful Methods in Molecular Medicine™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Interferon Methods and Protocols offers researchers powerful tools not only to ascertain the functions of IFN-stimulatory gene products, but also to identify additional molecular pathways that will clarify our understanding of the many biological events influenced by IFNs.

Editors and Affiliations

  • Department of Ophthalmology, The University of Oklahoma Health and Sciences Center, Oklahoma City

    Daniel J. J. Carr

Bibliographic Information

  • Book Title: Interferon Methods and Protocols

  • Editors: Daniel J. J. Carr

  • Series Title: Methods in Molecular Medicine

  • DOI: https://doi.org/10.1385/1592599397

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2005

  • Hardcover ISBN: 978-1-58829-418-0Published: 01 June 2005

  • Softcover ISBN: 978-1-61737-554-5Published: 10 November 2010

  • eBook ISBN: 978-1-59259-939-4Published: 02 February 2008

  • Series ISSN: 1543-1894

  • Series E-ISSN: 1940-6037

  • Edition Number: 1

  • Number of Pages: 240

  • Number of Illustrations: 50 b/w illustrations, 1 illustrations in colour

  • Topics: Immunology

  • Industry Sectors: Biotechnology, Consumer Packaged Goods, Health & Hospitals, Pharma

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