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RNA Silencing

Methods and Protocols

  • Gordon G. Carmichael

Part of the Methods in Molecular Biology™ book series (MIMB, volume 309)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Yoontae Lee, V. Narry Kim
    Pages 17-28
  3. Nathaniel R. Dudley, Bob Goldstein
    Pages 29-38
  4. Christine E. Clayton, Antonio M. Estévez, Claudia Hartmann, Vincent P. Alibu, Mark Field, David Horn
    Pages 39-59
  5. M. Florian Mette, Werner Aufsatz, Tatsuo Kanno, Lucia Daxinger, Philipp Rovina, Marjori Matzke et al.
    Pages 61-82
  6. Jason W. Myers, James E. Ferrell Jr.
    Pages 93-196
  7. Sung-Suk Chae, Timothy Hla
    Pages 197-203
  8. Guangchao Sui, Yang Shi
    Pages 205-218
  9. Scott Q. Harper, Beverly L. Davidson
    Pages 219-235
  10. Mouldy Sioud
    Pages 237-249
  11. Federica Simeoni, May C. Morris, Frederic Heitz, Gilles Divita
    Pages 251-260
  12. Alicia M. Celotto, Joo-Won Lee, Brenton R. Graveley
    Pages 273-282
  13. Elisavet Maniataki, Maria Dels Angels De Planell Saguer, Zissimos Mourelatos
    Pages 283-294
  14. Marianthi Kiriakidou, Peter Nelson, Stella Lamprinaki, Anup Sharma, Zissimos Mourelatos
    Pages 295-310
  15. Peng Liu, Mary Louise Stover, Alexander Lichtler, David W. Rowe
    Pages 321-331
  16. Back Matter
    Pages 333-338

About this book

Introduction

The past decade has witnessed a true revolution in our understanding of how RNA molecules can reduce or silence gene expression, a process that holds great promise in basic research and for antiviral and anticancer applications. In RNA Silencing: Methods and Protocols, many of the most notable and distinguished researchers in the field describe in step-by-step detail their best methods for the design, preparation, and use of RNAs to reduce gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and state-of-the-art, RNA Silencing: Methods and Protocols offers today's laboratory investigators a host of optimized and validated techniques to study RNA silencing and translate its concepts into successful practical applications.

Keywords

DNA Vivo gene expression genes molecular biology regulation transcription

Editors and affiliations

  • Gordon G. Carmichael
    • 1
  1. 1.Department of Genetics and Developmental BiologyUniversity of Connecticut Health CenterFarmington

Bibliographic information

  • DOI https://doi.org/10.1385/1592599354
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-436-4
  • Online ISBN 978-1-59259-935-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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