Microarrays in Clinical Diagnostics

  • Thomas O. Joos
  • Paolo Fortina

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 114)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Yilmaz Niyaz, Monika Stich, Bernd Sägmüller, Renate Burgemeister, Gabriele Friedemann, Ulrich Sauer et al.
    Pages 1-24
  3. Reyna Favis, Norman P. Gerry, Yu-Wei Cheng, Francis Barany
    Pages 25-58
  4. Lovisa Lovmar, Ann-Christine Syvänen
    Pages 79-92
  5. Maurizio Ferrari, Laura Cremonesi, Pierangelo Bonini, Barbara Foglieni, Stefania Stenirri
    Pages 93-106
  6. Achim Wixforth
    Pages 121-145
  7. Rhonda Bangham, Gregory A. Michaud, Barry Schweitzer, Paul F. Predki
    Pages 173-182
  8. Tito Bacarese-Hamilton, Julian Gray, Andrea Ardizzoni, Andrea Crisanti
    Pages 195-207
  9. Uma Prabhakar, Edward Eirikis, Bruce E. Miller, Hugh M. Davis
    Pages 223-232
  10. Ronald Simon, Martina Mirlacher, Guido Sauter
    Pages 257-268
  11. Back Matter
    Pages 269-274

About this book

Introduction

Microarray technology is now well established in laboratories all over the world, both for rapid detection of genetic mutations and, increasingly, for simultaneously analyzing the expression of a large number of proteins. In Microarrays in Clinical Diagnostics, a panel of academic and industrial investigators surveys the new world of microarray technology, describing in step-by-step detail diverse DNA and protein assays in clinical laboratories using state-of-the-art technologies. The advanced tools and methods described are designed for mRNA expression analysis, SNP analysis, identification and quantification of proteins, and for studies of protein-protein interactions. Among the highlights are universal arrays, antibody microarrays, direct labeling approaches, and quality control techniques for protein microarray-based analysis. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and highly practical, Microarrays in Clinical Diagnostics combines a comprehensive collection of cutting-edge technologies with detailed laboratory methods to help clinical laboratory researchers design and perform microarray experiments for DNA and protein analysis, diagnostic testing, and cancer research.

Keywords

DNA PCR SNP diagnostics genes hybridization

Editors and affiliations

  • Thomas O. Joos
    • 1
  • Paolo Fortina
    • 2
  1. 1.Biochemistry DepartmentNMI Natural and Medical Sciences Institute at the University of TuebingenReutlingenGermany
  2. 2.Center for Translational Medicine, Department of MedicineJefferson Medical CollegePhiladelphia

Bibliographic information

  • DOI https://doi.org/10.1385/1592599230
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-394-7
  • Online ISBN 978-1-59259-923-3
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • About this book
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