Peptide Synthesis and Applications

  • John Howl

Part of the Methods in Molecular Biology™ book series (MIMB, volume 298)

Table of contents

  1. Front Matter
    Pages i-x
  2. Common Strategies

    1. Front Matter
      Pages 1-1
    2. Muriel Amblard, Jean-Alain Fehrentz, Jean Martinez, Gilles Subra
      Pages 3-24
    3. John Howl
      Pages 25-41
  3. Synthetic Methodologies and Applications

    1. Front Matter
      Pages 43-43
    2. Joanne T. Blanchfield, Istvan Toth
      Pages 45-61
    3. Gábor Mezö, Ferenc Hudecz
      Pages 63-76
    4. Margus Pooga, lo Langel
      Pages 77-89
    5. Zuzana Machova, Annette G. Beck-Sickinger
      Pages 105-130
    6. Rebecca A. Lew, Nathalie Tochon-Danguy, Catherine A. Hamilton, Karen M. Stewart, Marie-Isabel Aguilar, A. Ian Smith
      Pages 143-150
    7. Gregory T. Bourne, Jonathon L. Nielson, Justin F. Coughlan, Paul Darwen, Marc R. Campitelli, Douglas A. Horton et al.
      Pages 151-166
    8. Michal Lebl, John Hachmann
      Pages 167-194
    9. Jordi Alsina, Steven A. Kates, George Barany, Fernando Albericio
      Pages 195-208
    10. Ferenc Hudecz
      Pages 209-223
  4. Practical Guides

    1. Front Matter
      Pages 225-225
    2. Ursel Soomets, Mihkel Zilmer, lo Langel
      Pages 241-257
  5. Back Matter
    Pages 259-262

About this book

Introduction

Peptide synthesis provides novel materials that can serve as both valuable research tools and candidate therapeutics. In Peptide Synthesis and Applications, a panel of expert researchers and established group leaders describe in step-by-step detail the key methodologies of contemporary peptide synthesis and illustrate numerous applications that employ peptides as unique and essential materials. The techniques presented include protocols for chemical ligation, the synthesis of cyclic and phosphotyrosine-containing peptides, lipoamino acid- and sugar-conjugated peptides, and peptide purification and analyses. Additional chapters detail methodologies and instrumentation for high-throughput peptide synthesis, many different applications of peptides as novel research tools and biological probes, and the design and application of fluorescent substrate-based peptides that can be used to determine the selectivity and activity of peptidases. A practical guide to the identification of proteins using mass spectrometric analyses of peptide mixtures is also included. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and highly practical, Peptide Synthesis and Applications offers novice and experienced investigators alike powerful techniques for the design and manufacture of their own peptide-based materials that can be used as productive research tools or developed as promising new therapeutics.

Keywords

Alanin Amino acid Ligation nucleic acid protein proteins signal transduction synthesis

Editors and affiliations

  • John Howl
    • 1
  1. 1.Research Institute in Healthcare ScienceSchool of Applied Sciences, University of WolverhamptonWolverhamptonUK

Bibliographic information

  • DOI https://doi.org/10.1385/1592598773
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-317-6
  • Online ISBN 978-1-59259-877-9
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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