Cell Migration

1. Analysis of Cell Migration in Model Organisms

   1. Analysis of Cell Movements in Zebrafish Embryos

      • Diane S. Sepich, Lilianna Solnica-Krezel

      Pages 211-233

   2. The Xenopus Embryo as a Model System for Studies of Cell Migration

      • Douglas W. DeSimone, Lance Davidson, Mungo Marsden, Dominique Alfandari

      Pages 235-245

   3. Neural Crest Migration Methods in the Chicken Embryo

      • Maria Elena de Bellard, Marianne Bronner-Fraser

      Pages 247-267

   4. In Vivo and In Vitro Models of Mammalian Angiogenesis

      • Mien V. Hoang, Donald R. Senger

      Pages 269-285

2. Biochemical and Novel Approaches in Cell Migration

   1. Front Matter

      Pages 287-287

      PDF

   2. Measurement of Protein Tyrosine Phosphorylation in Cell Adhesion

      • Vidhya V. Iyer, Christoph Ballestrem, Joachim Kirchner, Benjamin Geiger, Michael D. Schaller

      Pages 289-302

   3. Cell-Adhesion Assays

      • Frauke Drees, Amy Reilein, W. James Nelson

      Pages 303-320

   4. Application of Microscope-Based FRET to Study Molecular Interactions in Focal Adhesions of Live Cells

      • Christoph Ballestrem, Benjamin Geiger

      Pages 321-334

   5. Use of Multiphoton Imaging for Studying Cell Migration in the Mouse

      • Andrea Flesken-Nikitin, Rebecca M. Williams, Warren R. Zipfel, Watt W. Webb, Alexander Yu. Nikitin

      Pages 335-345

   6. Using Microfluidic Channel Networks to Generate Gradients for Studying Cell Migration

      • Daniel S. Rhoads, Sharvari M. Nadkarni, Loling Song, Camilla Voeltz, Eberhard Bodenschatz, Jun-Lin Guan

      Pages 347-357

3. Back Matter

   Pages 359-364

   PDF

A collection of classic, novel, and state-of-the-art methods for the study of cell migration in cultured cells, different model organisms, and specialized cells in normal development and disease. Highlights include basic assays that apply to all cell migration studies in vitro, assays in various model organisms, and assays for cancer cells, endothelial cells, and neurons both in vitro and in animal models. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

• Drosophila
• Vivo
• biology
• cancer
• cell
• development
• membrane
• microscopy
• migration
• molecular biology
• protein

• The Department of Molecular Medicine, College of Veterinary Medicine, Cornell University, Ithaca

  Jun-Lin Guan

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