Molecular Toxicology Protocols

  • Phouthone Keohavong
  • Stephen G. Grant

Part of the Methods in Molecular Biology™ book series (MIMB, volume 291)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Analysis of DNA Adducts

    1. Front Matter
      Pages 1-1
    2. David H. Phillips, Alan Hewer, Volker M. Arlt
      Pages 3-12
    3. Karen H. Dingley, Esther A. Ubick, John S. Vogel, Kurt W. Haack
      Pages 21-27
    4. Iovanna Pandelova, Stephen R. Hewitt, John B. Hays
      Pages 29-38
    5. Walter A. Deutsch, Vijay Hegde
      Pages 39-46
  3. Detection of Chromosomal and Genome-Wide Damage

    1. Front Matter
      Pages 47-47
    2. Xifeng Wu, Yun-Ling Zheng, T. C. Hsu
      Pages 59-67
    3. Günter Speit, Andreas Hartmann
      Pages 85-95
    4. Alexander Rapp, Michael Hausmann, Karl Otto Greulich
      Pages 107-119
  4. Detection and Characterization of Surrogate Gene Mutation

    1. Front Matter
      Pages 131-131
    2. Vasily N. Dobrovolsky, Joseph G. Shaddock, Robert H. Heflich
      Pages 133-144
    3. Phouthone Keohavong, Liqiang Xi, Stephen G. Grant
      Pages 161-170

About this book

Introduction

New state-of-the-art molecular techniques promise to transform the field of genetic toxicology by making it possible to directly detect genotoxic exposures and their consequences in humans, to identify the agent(s) involved, and to clinically manage the exposed population. In Molecular Toxicology Protocols, researchers from prominent universities and cancer centers around the world describe in detail their best techniques for analyzing genotoxic exposure and the resulting biological effects, including intermediate biomarkers such as DNA and chromosomal damage, mutations in reporter and oncogenes, and the earliest possible detection of cancer. The authors emphasize analytical methods specifically developed for use in human populations and in cancer patients, or in other in vivo systems such as transgenic mice. Among the applications detailed are the analysis of interactions of chemical and physical agents with cellular macromolecules, especially DNA, the assessment of medically relevant toxicity, and the individualized characterization of genetic damage and repair. There are also methods to assess and characterize the modulation of this damage and repair through individual differences in specific and genome-wide gene expression, including metabolic profiling and apoptotic capacity. These methods mark a shift in emphasis from studies of the agents themselves to the exposed population, and from studies of small populations with significant known exposures to a single agent, to studies of common diseases, such as breast cancer, caused by normal levels of generalized genotoxic exposure. The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and highly practical, Molecular Toxicology Protocols offers a gold-standard collection of cutting-edge techniques designed to investigate a broad range of exposures-endogenous, accidental, medical, environmental, and occupational-and their role in human carcinogenesis and other diseases of aging.

Keywords

Allele DNA FISH Ligation PCR cancer risk chromium gene expression genes hybridization toxicology transgenic animals

Editors and affiliations

  • Phouthone Keohavong
    • 1
  • Stephen G. Grant
    • 1
  1. 1.Department of Environmental and Occupational HealthUniversity of PittsburghPittsburgh

Bibliographic information

  • DOI https://doi.org/10.1385/1592598404
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-084-7
  • Online ISBN 978-1-59259-840-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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