Basic Cell Culture Protocols

  • Cheryl D. Helgason
  • Cindy L. Miller

Part of the Methods in Molecular Biology™ book series (MIMB, volume 290)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Cord C. Uphoff, Hans G. Drexler
    Pages 13-23
  3. Cord C. Uphoff, Hans G. Drexler
    Pages 25-34
  4. Wilhelm G. Dirks, Hans G. Drexler
    Pages 35-50
  5. Roderick A. F. MacLeod, Hans G. Drexler
    Pages 51-70
  6. Cindy L. Miller, Becky Lai
    Pages 71-89
  7. John Q. Davies, Siamon Gordon
    Pages 91-103
  8. John Q. Davies, Siamon Gordon
    Pages 105-116
  9. Tomoo Ueno, Cunlan Liu, Takeshi Nitta, Yousuke Takahama
    Pages 117-133
  10. Renée F. de Pooter, Sarah K. Cho, Juan Carlos Zúñiga-Pflücker
    Pages 135-147
  11. Xinghui Tian, Dan S. Kaufman
    Pages 149-162
  12. Robert A. J. Oostendorp, Kirsty Harvey, Elaine A. Dzierzak
    Pages 163-172
  13. Frizell L. Vaughan, Ludmila I. Bernstam
    Pages 187-206
  14. Edward L. LeCluyse, Eliane Alexandre, Geraldine A. Hamilton, Catherine Viollon-Abadie, D. James Coon, Summer Jolley et al.
    Pages 207-229
  15. Mary Taub
    Pages 231-247
  16. John Stingl, Joanne T. Emerman, Connie J. Eaves
    Pages 249-263

About this book

Introduction

In this fully revised edition of an established classic, expert researchers and clinicians describe in step-by-step detail updated techniques for the isolation and growth of the major primary cell types, such as kidney proximal tubule cells, hepatocytes, keratinocytes, and cardiomyocytes. The authors offer readily reproducible new methods for the differentiation of embryonic stem (ES) cells into various hematopoietic cell types, for fetal thymic organ culture, and for the isolation and culture of specialized cell types, such as mammary progenitor cells, skeletal muscle myofibers, mesenchymal cells, neural stem cells, hematopoietic cells, stromal cell lines, and endothelial cells. Additional chapters describe new techniques (leukocyte rolling, isolation of side population cells, and scalable production of ES-derived cells) and detail quality control methods for cell lines (detection and elimination of mycoplasma, DNA fingerprinting, and cytogenetic analysis). The protocols follow the successful Methods in Molecular Biology™ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Up-to-date and highly practical, Basic Cell Culture Protocols, Third Edition, offers basic scientists and clinician-researchers powerful tools to isolate, culture, and characterize the promising specialized cell types in demand today.

Keywords

DNA Macrophages cell cell culture cell lines development enzyme human cell lines neural stem cells stem cell stem cells tissue

Editors and affiliations

  • Cheryl D. Helgason
    • 1
  • Cindy L. Miller
    • 2
  1. 1.Department of Cancer EndocrinologyBritish Columbia Cancer AgencyVancouverCanada
  2. 2.StemCell Technologies Inc.VancouverCanada

Bibliographic information

  • DOI https://doi.org/10.1385/1592598382
  • Copyright Information Humana Press 2005
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-284-1
  • Online ISBN 978-1-59259-838-0
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
Industry Sectors
Biotechnology
Consumer Packaged Goods
Pharma