Epidermal Cells

1. Analysis of Epidermal Differentiation

   1. Analysis of E2F Factors During Epidermal Differentiation

      • Wing Y. Chang, Lina Dagnino

      Pages 147-156

   2. Analysis of HOX Homeodomain Proteins and Gene Transcripts in the Epidermis

      • László G. Kömüves, Corey Largman

      Pages 157-169

   3. Apoptosis in the Epidermis

      • Kiyofumi Yamanishi, Chun-Shen Shen, Hitoshi Mizutani

      Pages 171-174

   4. Fate of Desmosomal Proteins in Apoptotic Epidermal Cells

      • Jörg Weiske, Otmar Huber

      Pages 175-191

   5. Analysis of Connexin 43 Expression on Keratinocytes Using Flow Cytometry

      • Maja Matic, Christopher Pullis, Marc G. Golightly, Sanford R. Simon

      Pages 193-199

   6. MMP-9 and TIMP-1 Assays in Keratinocyte Cultures

      • Takashi Kobayashi

      Pages 201-208

   7. Characterization of Epithelial Cells in the Hair Follicle With S100 Proteins

      • Kenji Kizawa, Mayumi Ito

      Pages 209-221

   8. Immunoelectron Microscopic Analysis of Cornified Cell Envelopes and Antigen Retrieval

      • Akemi Ishida-Yamamoto

      Pages 223-226

2. Methods and Approaches for the Analysis for Epidermal Function

   1. Front Matter

      Pages 227-227

      PDF

   2. Cell Kinetic Analysis in Artificial Skin Using Immunochemical Methods

      • Andrea Casasco, Antonia Icaro Cornaglia, Federica Riva, Marco Casasco, Alberto Calligaro

      Pages 229-238

   3. Proliferation, Differentiation, and Inflammation in Normal and Hyperproliferative Skin Using Multiparameter Flow Cytometry

      • Piet E. J. van Erp

      Pages 239-250

   4. Fluorimetric DNA Assay of Cell Number

      • William R. Otto

      Pages 251-262

   5. Keratinocyte Transient Transfections

      • Anthony M. Flores, Brian J. Aneskievich

      Pages 263-272

   6. Tetracycline-Regulated Gene Expression in Epidermal Keratinocytes

      • Richard B. Presland, Philip Fleckman

      Pages 273-286

   7. Gene Targeting by Oligonucleotides in Keratinocytes

      • Olga Igoucheva, Kyonggeun Yoon

      Pages 287-302

   8. Promoter Analysis in the Human SPRR Gene Family

      • David F. Fischer, Claude Backendorf

      Pages 303-313

   9. Stable Integration of Large PAC Constructs in Keratinocytes

      • Sarah H. Williams, Alain Hovnanian

      Pages 315-328

   10. Targeted Somatic Mutagenesis in the Mouse Epidermis

       • Daniel Metzger, Mei Li, Pierre Chambon

       Pages 329-340

   11. Methods to Study Protein-Protein Interactions

       • Jin-Jun Meng, Meghan Rojas, Willis Bacon, John T. Stickney, Wallace Ip

       Pages 341-357

   12. Isolation of Recombinant Phage-Displayed Antibodies Recognizing Skin Keratinocytes

       • Kim Bak Jensen, Peter Kristensen

       Pages 359-370

A collection of cutting-edge methods to analyze and manipulate epidermal cellprecursors and mature epidermal cells. These protocols cover different methods and models for culturing epidermal cells, for enriching very early epidermal progenitors, and for studying epidermal cell commitment and differentiation both in vitro and in vivo. Topics of special interest include the derivation, characterization, and utility of epidermal stem cells, mature epidermal cells and their characterization, and applications in regenerative medicine. These readily reproducible techniques broaden our understanding of the biology of epidermal cells and of their utility in normal tissue homeostasis and regenerative medicine applications.

• DNA
• PAC
• PCR
• Promoter
• gene expression
• genes
• reproducible techniques

"The protocols are well written and fully detailed; they combine basic science protocols with informative diagrams illustrating results...will become an invaluable reference guide for laboratory researchers interested in mammalian epidermal cells." Doody's Health Sciences Book Review Journal

"For anyone who is planning or is already practically involved in experimental dermatological research, this impressive book is a must in the laboratory." -Acta Dermato-Venereologica

 

• Ottawa Health Research Institute, Ottawa, Canada

  Kursad Turksen

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