Transgenic Plants: Methods and Protocols

  • Leandro Peña

Part of the Methods in Molecular Biology™ book series (MIMB, volume 286)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Luis Herrera-Estrella, June Simpson, Miguel Martínez-Trujillo
      Pages 3-31
  3. Transformation

    1. Front Matter
      Pages 33-33
    2. Abhaya M. Dandekar, Henry J. Fisk
      Pages 35-46
    3. Julie R. Kikkert, José R. Vidal, Bruce I. Reisch
      Pages 61-78
    4. Henry Daniell, Oscar N. Ruiz, Amit Dhingra
      Pages 111-137
  4. Regeneration

    1. Front Matter
      Pages 139-139
    2. Anne Frary, Joyce Van Eck
      Pages 141-150
    3. Krystyna Klimaszewska, Robert G. Rutledge, Armand Séguin
      Pages 151-163
    4. Peng Zhang, Johanna Puonti-Kaerlas
      Pages 165-176
    5. Magdalena Cervera, José Juárez, Luis Navarro, Leandro Peña
      Pages 177-187
  5. Selection

    1. Front Matter
      Pages 189-189
    2. Julia L. Goodwin, Gabriela M. Pastori, Michael R. Davey, Huw D. Jones
      Pages 191-201
    3. Matthew D. Halfhill, Reginald J. Millwood, C. Neal Stewart Jr.
      Pages 215-225

About this book

Introduction

Transgenic plants serve as essential tools for research in plant biology and are the basis of modern agricultural biotechnology. In Transgenic Plants: Methods and Protocols, leading scientists describe readily reproducible techniques they have perfected for the genetic transformation and regeneration of plants. The authors cover the most commonly used transformation systems, including Agrobacterium tumefaciens, Agrobacterium rhizogenes, particle bombardment, electroporation, floral-dip, and chloroplast transformation, as well as the regeneration of whole transgenic plants by organogenesis and somatic embryogenesis from different explant cells/tissues and from such diverse plant species as tomato, cassava, conifers, and citrus. Also described are the use of selectable and reporter markers, positive selection (for maize transformation), and the controlled excision and removal of marker genes from both nuclei and plastids once transgenic shoots have been efficiently generated. Additional techniques employ quantitative real-time PCR, reverse transcription PCR, fluorescence in situ hybridization, and thermal asymmetric interlaced PCR. Risk assessment methods offer the opportunity to study Agrobacterium persistence in plant tissues and to investigate the possibility of transgene dispersal. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and easy to use, Transgenic Plants: Methods and Protocols offers both novice and experienced researchers a cutting-edge laboratory manual covering all aspects of plant genetic transformation and transgenic crops.

Editors and affiliations

  • Leandro Peña
    • 1
  1. 1.Instituto Valenciano de Investigaciones AgrariasValenciaSpain

Bibliographic information

  • DOI https://doi.org/10.1385/1592598277
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-263-6
  • Online ISBN 978-1-59259-827-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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