Photosynthesis Research Protocols

1. Extraction of the Functional Manganese and Calcium From Photosystem II

   • Joel Freeman, Garth Hendry, Tom Wydrzynski

   Pages 205-215

2. Assay of Photoinhibition of Photosystem II and Protease Activity

   • Yasusi Yamamoto, Yoji Nishi, Hitoshi Yamasaki, Suguru Uchida, Satoshi Ohira

   Pages 217-227

3. Thermoluminescence

   • Prafullachandra V. Sane

   Pages 229-248

4. Detection of Free Radicals and Reactive Oxygen Species

   • Éva Hideg

   Pages 249-260

5. Stabilization of Photosynthetic Materials

   • Régis Rouillon, Pierre Euzet, Robert Carpentier

   Pages 261-269

6. Determination of Phosphoproteins in Higher Plant Thylakoids

   • Eva-Mari Aro, Anne Rokka, Alexander V. Vener

   Pages 271-285

7. Identifying Photoprotection Mutants in Arabidopsis thaliana

   • Jan Bart Rossel, Abby Cuttriss, Barry J. Pogson

   Pages 287-299

8. A Simple Method for Chloroplast Transformation in Chlamydomonas reinhardtii

   • Velupillai M. Ramesh, Scott E. Bingham, Andrew N. Webber

   Pages 301-307

9. The Construction of Gene Knockouts in the Cyanobacterium Synechocystis sp. PCC 6803

   • Julian J. Eaton-Rye

   Pages 309-324

10. Gene Inactivation in the Cyanobacterium Synechococcus sp. PCC 7002 and the Green Sulfur Bacterium Chlorobium tepidum Using In Vitro-Made DNA Constructs and Natural Transformation

    • Niels-Ulrik Frigaard, Yumiko Sakuragi, Donald A. Bryant

    Pages 325-340

11. Back Matter

    Pages 341-344

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Photosynthesis is one of the most important biological phenomena on earth. The conversion of sunlight by photosynthetic organisms supplies most of the energy required to develop and sustain life on the planet. Photosynthesis is not only at the heart of plant bioenergetics, it is also fundamental to plant prod- tivity and biomass. Photosynthetic carbon fixation and oxygen evolution - rectly intervene in many environmental, including the global atmospheric CO 2 level and global climate. Therefore, it is not surprising that a large effort is devoted to photosynthesis research. Several biochemical methods of isolation, treatment, and analysis have been developed to fulfill the needs of photosynthesis research. Photosynthesis Research Protocols contains a broad range of general and fundamental me- ods that are commonly used by plant biochemists, physiologists, and mole- lar biologists. This book is thus intended as a source of information for scientists working on any of the multiple aspects of photosynthesis, and should be of great interest to a multidisciplinary field of research involving agric- ture, biochemistry, biotechnology, botany, cell biology, environmental s- ences, forestry, plant genetics, plant molecular biology, photobiology, photophysics, photoprotection, plant physiology, plant stress, etc.

From the reviews:

"This book series is well known as a competent tool for research protocols in all fields of life science. … The book addresses either beginners in photosynthesis research or experienced researchers who want to extend their methodological experience. … I can recommend the book as basic literature for advanced courses in photosynthesis. … In conclusion, this is an excellent book which can be recommended for all laboratories involved in photosynthesis education and research." (Christian Wilhelm, Journal of Plant Physiology, Vol. 162 (6), 2005)

• Départment de Chimie-Biologie (GREIB), Université du Québec à Trois-Rivières, Trois-Rivières, Canada

  Robert Carpentier

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