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B Cell Protocols

  • Book
  • © 2004

Overview

Editors:
  1. Hua Gu

    1. Department of Microbiology, Columbia University College of Physicians and Surgeons, New York

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    You can also search for this editor in PubMed   Google Scholar

  2. Klaus Rajewsky

    1. The Center for Blood Research, The Harvard Medical School, Boston

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

Part of the book series: Methods in Molecular Biology (MIMB, volume 271)

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Table of contents (19 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Characterization of B Lymphopoiesis in Mouse Bone Marrow and Spleen

    • Richard R. Hardy, Susan A. Shinton
    Pages 1-24

  3. Characterization of B-Cell Maturation in the Peripheral Immune System

    • Rita Carsetti
    Pages 25-36

  4. Identification of B-Cell Subsets

    • James W. Tung, David R. Parks, Wayne A. Moore, Leonard A. Herzenberg, Leonore A. Herzenberg
    Pages 37-58

  5. Analysis of B-Cell Life-Span and Homeostasis

    • Irmgard Föster
    Pages 59-66

  6. Differentiation of B Lymphocytes From Hematopoietic Stem Cells

    • Paulo Vieira, Ana Cumano
    Pages 67-76

  7. Analysis of Lymphocyte Development and Function Using the RAG-Deficient Blastocyst Complementation System

    • Faith M. Young, Carl A. Pinkert, Andrea Bottaro
    Pages 77-90

  8. Conditional Gene Mutagenesis in B-Lineage Cells

    • Stefano Casola
    Pages 91-109

  9. Analysis of the Germinal Center Reaction and In Vivo Long-Lived Plasma Cells

    • Kai-Michael Toellner, Mahmood Khan, Daniel Man-Yuen Sze
    Pages 111-125

  10. T-Cell-Dependent Immune Responses, Germinal Center Development, and the Analysis of V-Gene Sequences

    • Claudia Berek
    Pages 127-138

  11. Introduction of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors

    • Kuo-I Lin, Kathryn Calame
    Pages 139-148

  12. Immunoglobulin Class Switching

    • Sven Kracker, Andreas Radbruch
    Pages 149-159

  13. In Vitro and In Vivo Assays of B-Lymphocyte Migration

    • Chantal Moratz, John H. Kehrl
    Pages 161-171

  14. Analysis of Antigen-Specific B-Cell Memory Directly Ex Vivo

    • Louise J. McHeyzer-Williams, Michael G. McHeyzer-Williams
    Pages 173-188

  15. B-Cell Signal Transduction

    • Tilman Brummer, Winfried Elis, Michael Reth, Michael Huber
    Pages 189-212

  16. Isolation of Lipid Rafts From B Lymphocytes

    • Anu Cherukuri, Shiang-Jong Tzeng, Arun Gidwani, Hae Won Sohn, Pavel Tolar, Michelle D. Snyder et al.
    Pages 213-224

  17. Molecular Single-Cell PCR Analysis of Rearranged Immunoglobulin Genes As a Tool to Determine the Clonal Composition of Normal and Malignant Human B Cells

    • Ralf Küppers
    Pages 225-238

  18. Analysis of B-Cell Immune Tolerance Induction Using Transgenic Mice

    • Helen Ferry, Richard J. Cornall
    Pages 239-260

  19. Analysis of B-Cell Signaling Using DT40 B-Cell Line

    • Tomoharu Yasuda, Tadashi Yamamoto
    Pages 261-270

  20. B-Cell Development and Pre-B-1 Cell Plasticity In Vitro

    • Antonius G. Rolink
    Pages 271-281
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About this book

B-lymphocyte development and function remains an exciting area of research for those interested in the physiology and pathology of the immune system in higher animals. While recent advances in genetics and cellular and molecular biology have provided a large spectrum of powerful new experimental tools in this field, it is both time consuming and often very difficult for a student or just any bench-side worker to identify a reliable experimental protocol in the ocean of the literature. The aim of B Cell Protocols is to provide a collection of diverse protocols ranging from the latest inventions and applications to some classic, but still frequently used methods in B-cell biology. The authors of the various chapters are all highly qualified scientists who are either the inventors or expert users of these methods. Their extensive experience in mastering a particular method provides not only the step-by-step details of a reproducible protocol, but also useful troubleshooting tips that readers will appreciate in their daily work. We hope that this book will be helpful for both beginning and experienced researchers in the field in designing or modifying an experimental approach, and exploring a biological question from multiple angles.

Editors and Affiliations

  • Department of Microbiology, Columbia University College of Physicians and Surgeons, New York

    Hua Gu

  • The Center for Blood Research, The Harvard Medical School, Boston

    Klaus Rajewsky

Bibliographic Information

  • Book Title: B Cell Protocols

  • Editors: Hua Gu, Klaus Rajewsky

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592597963

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • Hardcover ISBN: 978-1-58829-347-3Published: 12 May 2004

  • Softcover ISBN: 978-1-61737-510-1Published: 19 November 2010

  • eBook ISBN: 978-1-59259-796-3Published: 04 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XII, 292

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Pharma

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