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  • © 2004

Platelets and Megakaryocytes

Volume 2: Perspectives and Techniques

Part of the book series: Methods in Molecular Biology (MIMB, volume 273)

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  • ISBN: 978-1-59259-783-3
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Softcover Book USD 249.99
Price excludes VAT (USA)
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Table of contents (29 protocols)

  1. Front Matter

    Pages i-xix
  2. Perspectives

    1. Front Matter

      Pages 1-1
    2. Signaling Receptors on Platelets and Megakaryocytes

      • Donna Woulfe, Jing Yang, Nicolas Prevost, Peter O’Brien, Ryan Fortna, Massimiliano Tognolini et al.
      Pages 3-31
    3. Manipulation of Gene Expression in Megakaryocytes

      • Nikla R. Emambokus, George J. Murphy, Jonathan Frampton
      Pages 33-55
    4. In Vitro Changes of Platelet Parameters

      • José Rivera, María L. Lozano, Vicente Vicente
      Pages 57-72
    5. Inhibitors of Cellular Signaling Targets

      • Chris J. Vlahos, Louis F. Stancato
      Pages 87-102
  3. Signaling Techniques

    1. Front Matter

      Pages 103-103
    2. Ligand-Binding Assays for Collagen

      • Stephanie M. Jung, Masaaki Moroi
      Pages 105-114
    3. Ligand-Binding Assays

      • Dermot Cox
      Pages 125-137
    4. Protein Tyrosine Phosphatases

      • Matthew L. Jones, Alastair W. Poole
      Pages 169-177
    5. The Study of Serine-Threonine Kinases

      • Gertie Gorter, Jan Willem Akkerman
      Pages 179-199
    6. Phosphoinositides

      • Bernard Payrastre
      Pages 201-212
    7. Isolation and Analysis of Platelet Lipid Rafts

      • Corie N. Shrimpton, Karine Gousset, Fern Tablin, José A. López
      Pages 213-228
    8. Measurement and Manipulation of [Ca2+]i in Suspensions of Platelets and Cell Cultures

      • Philippe Ohlmann, Béatrice Hechler, Jean-Pierre Cazenave, Christian Gachet
      Pages 229-250

About this book

Platelets are an essential element of the body's hemostasis system, and yet, through their involvement in thrombosis, are also a major cause of morbidity and mortality. Platelets have a limited lifespan and must be continually manufactured by their precursor, the megakaryocyte. In Platelets and Megakaryocytes, Volume 2: Perspectives and Techniques leading expert researchers-many responsible for seminal discoveries in the field-offer basic and advanced techniques for studying cell signaling in the regulation and function of platelets and megakaryocytes, as well as state-of-the-art commentaries on our understanding of these cells. The methods include ligand-binding assays, the study of protein and lipid kinases and phosphatases, the analysis of lipid rafts in the regulation of cell signaling, and the measurement of intracellular calcium levels. There are also techniques for the study of electrophysiological events, nitric oxide signaling, venom proteins, and for the internalization of proteins into platelets through permeabilization. The application of many molecular and post-genomic methods to the study of platelets and megakaryocytes is also described. All protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. A companion volume, Platelets and Megakaryocytes, Volume 1: Functional Assays describes in step-by-step detail basic and advanced techniques for analyzing platelet and megakaryocyte function.
Comprehensive and cutting-edge, the two volumes of Platelets and Megakaryocytes offer novice and experienced cell biologists, hematologists, and clinicians not only a benchmark survey of the field, but also a comprehensive library of proven techniques essential for productive research on platelets and megakaryocytes today.

Keywords

  • Activation
  • Proteomics
  • Viruses
  • Western Blotting
  • cell lines
  • gene expression
  • genes
  • microscopy
  • molecular biology
  • platelets
  • proteins

Reviews

"...thorough and detailed...contains useful information regarding the study of platelets and megakaryocytes." - Veterinary Pathology

Editors and Affiliations

  • School of Animal and Microbial Sciences, The University of Reading, Reading, UK

    Jonathan M. Gibbins

  • Department of Physiology, University of Cambridge, Cambridge, UK

    Martyn P. Mahaut-Smith

Bibliographic Information

  • Book Title: Platelets and Megakaryocytes

  • Book Subtitle: Volume 2: Perspectives and Techniques

  • Editors: Jonathan M. Gibbins, Martyn P. Mahaut-Smith

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592597831

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • Hardcover ISBN: 978-1-58829-011-3

  • Softcover ISBN: 978-1-61737-305-3

  • eBook ISBN: 978-1-59259-783-3

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XX, 504

  • Number of Illustrations: 108 b/w illustrations, 5 illustrations in colour

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Health & Hospitals, Pharma

Buying options

eBook USD 189.00
Price excludes VAT (USA)
  • ISBN: 978-1-59259-783-3
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 249.99
Price excludes VAT (USA)
Hardcover Book USD 279.99
Price excludes VAT (USA)