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  • © 2004

Recombinant Gene Expression

Reviews and Protocols

Part of the book series: Methods in Molecular Biology (MIMB, volume 267)

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  • ISBN: 978-1-59259-774-1
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Table of contents (33 protocols)

  1. Front Matter

    Pages i-xvi
  2. General Issues About Recombinant Gene Expression

    1. Front Matter

      Pages 1-1
    2. Production of Recombinant Proteins

      • Laura A. Palomares, Sandino Estrada-Moncada, Octavio T. Ramírez
      Pages 15-51
  3. Prokaryotes

    1. Front Matter

      Pages 75-75
    2. Back to Basics

      • Paulina Balbás, Francisco Bolívar
      Pages 77-90
    3. Expression of Recombinant Alkaline Phosphatase Conjugates in Escherichia coli

      • Jean-Claude Boulain, Frédéric Ducancel
      Pages 101-112
    4. Overexpression of Chromosomal Genes in Escherichia coli

      • Fernando Valle, Noemí Flores
      Pages 113-122
    5. Plasmid Vectors for Marker-Free Chromosomal Insertion of Genetic Material in Escherichia coli

      • Sylvie Le Borgne, Francisco Bolívar, Guillermo Gosset
      Pages 135-143
    6. Copy-Control pBAC/oriV Vectors for Genomic Cloning

      • Jadwiga Wild, Waclaw Szybalski
      Pages 145-154
    7. Copy-Control Tightly Regulated Expression Vectors Based on pBAC/oriV

      • Jadwiga Wild, Waclaw Szybalski
      Pages 155-167
    8. Cell-Free Protein Synthesis With Prokaryotic Combined Transcription-Translation

      • James R. Swartz, Michael C. Jewett, Kim A. Woodrow
      Pages 169-182
    9. Gene Transfer and Expression of Recombinant Proteins in Moderately Halophilic Bacteria

      • Amalia S. Afendra, Carmen Vargas, Joaquín J. Nieto, Constantin Drainas
      Pages 209-223
    10. Recombinant Protein Production in Antarctic Gram-Negative Bacteria

      • Angela Duilio, Maria Luisa Tutino, Gennaro Marino
      Pages 225-237
  4. Fungi

    1. Front Matter

      Pages 239-239
    2. Recombinant Protein Production in Yeasts

      • Danilo Porro, Diethard Mattanovich
      Pages 241-258

About this book

In this updated and expanded second edition of an established classic, the editors have added critical reviews to a fresh collection of cutting-edge protocols for gene expression in bacteria, fungi, plants, plant cells, animals, and animal cells. The review articles survey new directions in recombinant gene expression research, technique, and application, and point the way to using recombinant gene expression for metabolic engineering and the production of nonprotein molecules. The readily reproducible protocols offer new host-vector systems, such as Antarctic bacteria and moderately halophytic bacteria, vectors for chromosomal editing and metabolic engineering, and information to aid in problem solving, strategy planning, and the promotion of protein folding. There are also novel heterologous gene expression schemes for cell-free systems, plant chloroplasts, microplates, hairy roots, and animal milk, and new developments in consolidated heterologous systems. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and forward looking, Recombinant Gene Expression: Reviews and Protocols, Second Edition offers investigators seeking an overview of this critically important field not only the understanding, but also the tools needed to begin producing nonprotein products and altering the central metabolic pathways of cells to enhance heterologous gene expression.

Reviews

From reviews of the first edition...
"...particularly useful for teaching molecular biology laboratory courses."
-Quarterly Review of Biology

"...represents good value and will be a useful addition to the laboratory."
-Microbiology Today

Editors and Affiliations

  • Centro de Investigación en Biotecnología, UAEM, Cuernavaca, México

    Paulina Balbás

  • Virginia Polytechnic Institute and State University, Blacksburg

    Argelia Lorence

Bibliographic Information

  • Book Title: Recombinant Gene Expression

  • Book Subtitle: Reviews and Protocols

  • Editors: Paulina Balbás, Argelia Lorence

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592597742

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • Hardcover ISBN: 978-1-58829-262-9

  • Softcover ISBN: 978-1-61737-460-9

  • eBook ISBN: 978-1-59259-774-1

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XVI, 508

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Health & Hospitals, Pharma

Buying options

eBook USD 149.00
Price excludes VAT (USA)
  • ISBN: 978-1-59259-774-1
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book USD 199.00
Price excludes VAT (USA)
Hardcover Book USD 249.99
Price excludes VAT (USA)