Public Health Microbiology

Methods and Protocols

  • John F. T. Spencer
  • Alicia L. Ragout de Spencer

Part of the Methods in Molecular Biology book series (MIMB, volume 268)

Table of contents

  1. Front Matter
    Pages i-xvii
  2. Bacteria

    1. Front Matter
      Pages 1-1
    2. Rustam I. Aminov, Joanne C. Chee-Sanford, Natalie Garrigues, Asma Mehboob, Roderick I. Mackie
      Pages 3-13
    3. Brigid Lucey, Fiona O’Halloran, Séamus Fanning
      Pages 33-47
    4. Ana Belén Vivanco, Juan Alvarez, Idoia Laconcha, Nuria López-Molina, Aitor Rementeria, Javier Garaizar
      Pages 49-57
  3. Viruses

    1. Front Matter
      Pages 59-59
    2. Albert Bosch, Rosa M. Pintó, Jaume Comas, Francesc-Xavier Abad
      Pages 61-68
    3. Fiona O’Halloran, Séamus Fanning
      Pages 89-102
    4. Glòria Sánchez, Cristina Villena, Albert Bosch, Rosa M. Pintó
      Pages 103-114
  4. Fungi

    1. Front Matter
      Pages 115-115
    2. Aitor Rementeria, Ana Belén Vivanco, Ainara Cadaval, María Teresa Ruesga, Sonia Brena, José Pontón et al.
      Pages 117-125
    3. Dante Javier Bueno, Julio Oscar Silva, Guillermo Oliver
      Pages 127-131
    4. Dante Javier Bueno, Guillermo Oliver
      Pages 133-137
  5. Other Pathogens

    1. Front Matter
      Pages 139-139
    2. Judith A. Noble-Wang, Shangtong Zhang, Daniel Price, Donald G. Ahearn
      Pages 153-161
    3. Lihua Xiao, Altaf A. Lal, Jianlin Jiang
      Pages 163-176

About this book


The world abounds with microorganisms that are hazardous to human health in the normal course of life. In Public Health Microbiology: Methods and Protocols, expert investigators describe in step-by-step detail the laboratory techniques they have perfected for determining the nature and character of a diverse group of hazardous bacteria, viruses, and fungi. For bacterial and viral pathogens, the authors give special attention to PCR methods for detecting genes resistant to tetracycline, for resistance in Salmonella enterica, for identifying and typing Campylobacter coli, and for detecting the abundance of enteric, hepatitis A, and rotaviruses in sewage, as well as bacteriophages infecting the O157:H7 strain of Escherichia coli. For fungi the authors offer methods for computerized analysis and typing of fungal isolates, for the isolation and enumeration of fungi in foods, and for the determination of aflatoxin and zearalenone. Other methods deal with hazard analysis, the use of disinfectants, microbiological analysis of cosmetics, microbiological tests for sanitation equipment in factories, and the uses of animals in studies of various characteristics of lactobacilli. Review articles address the spread of pathogens from livestock and poultry production, including the threat of prion-based diseases. All protocols presented follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Timely and highly practical, Public Health Microbiology: Methods and Protocols offers researchers and public health specialists alike a wide-ranging collection of methods for the determination of many important pathogenic microorganisms, their effects on health, and possible measures that can be taken to counter these effects.

Editors and affiliations

  • John F. T. Spencer
    • 1
  • Alicia L. Ragout de Spencer
    • 1
  1. 1.Planta Piloto de Procesos Industriales Microbiológicos(PROIMI)-CONICETSan Miguel de TucumánArgentina

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-117-2
  • Online ISBN 978-1-59259-766-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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