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  • Book
  • © 2004

Genetic Recombination

Reviews and Protocols

Editors:

  1. Alan S. Waldman

    1. Department of Biological Sciences, University of South Carolina, Coumbia

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Part of the book series: Methods in Molecular Biology (MIMB, volume 262)

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Table of contents (15 protocols)

  1. Front Matter

    Pages i-x
    PDF

  2. Studying Recombination Events in Eukaryotes

    1. Front Matter

      Pages 1-1
      PDF

    4. Intrachromosomal Homologous Recombination in Arabidopsis thaliana

      • Waltraud Schmidt-Puchta, Nadiya Orel, Anzhela Kyryk, Holger Puchta
      Pages 25-34

    6. Transformation of Monomorphic and Pleomorphic Trypanosoma brucei

      • Richard McCulloch, Erik Vassella, Peter Burton, Michael Boshart, J. David Barry
      Pages 53-86

    7. Forward Genetic Screens for Meiotic and Mitotic Recombination-Defective Mutants in Mice

      • Laura Reinholdt, Terry Ashley, John Schimenti, Naoko Shima
      Pages 87-107

  3. Recombination as a Reporter of Genomic Instability

    1. Front Matter

      Pages 109-109
      PDF

    2. Detecting Carcinogens With the Yeast DEL Assay

      • Richard J. Brennan, Robert H. Schiestl
      Pages 111-124

    3. In Vivo DNA Deletion Assay to Detect Environmental and Genetic Predisposition to Cancer

      • Ramune Reliene, Alexander J. R. Bishop, Jiri Aubrecht, Robert H. Schiestl
      Pages 125-139

  4. Recombination as a Tool for Producing Targeted Genetic Modification

    1. Front Matter

      Pages 141-141
      PDF

    3. DNA Fragment Transplacement in Saccharomyces cerevisiae

      • Glenn M. Manthey, Michelle S. Navarro, Adam M. Bailis
      Pages 157-172

    4. Targeted Gene Modification Using Triplex-Forming Oligonucleotides

      • Jean Y. Kuan, Peter M. Glazer
      Pages 173-194

  5. Biochemistry of Recombination

    1. Front Matter

      Pages 221-221
      PDF

    3. Holliday Junction Branch Migration and Resolution Assays

      • Angelos Constantinou, Stephen C. West
      Pages 239-253
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About this book

Genetic recombination, in the broadest sense, can be defined as any process in which DNA sequences interact and undergo a transfer of information, producing new “recombinant” sequences that contain information from each of the original molecules. All organisms have the ability to carry out recombination, and this striking universality speaks to the essential role recombination plays in a variety of biological processes fundamentally important to the maintenance of life. Such processes include DNA repair, regulation of gene expression, disease etiology, meiotic chromosome segregation, and evolution. One important aspect of recombination is that it typically occurs only between sequences that display a high degree of sequence identity. The stringent requirement for homology helps to ensure that, under normal circumstances, a cell is protected from deleterious rearrangements since a swap of genetic information between two nearly identical sequences is not expected to dramatically alter a genome. Recombination between dissimilar sequences, which does happen on occasion, may have such harmful consequences as chromosomal translocations, deletions, or inversions. For many organisms, it is also important that recombination rates are not too high lest the genome become destabilized. Curiously, certain organisms, such as the trypanosome parasite, actually use a high rate of recombination at a particular locus in order to switch antigen expression continually and evade the host immune system effectively.
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Editors and Affiliations

  • Department of Biological Sciences, University of South Carolina, Coumbia

    Alan S. Waldman

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Bibliographic Information

  • Book Title: Genetic Recombination

  • Book Subtitle: Reviews and Protocols

  • Editors: Alan S. Waldman

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592597610

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2004

  • Hardcover ISBN: 978-1-58829-236-0Published: 09 January 2004

  • Softcover ISBN: 978-1-61737-442-5Published: 10 November 2010

  • eBook ISBN: 978-1-59259-761-1Published: 03 February 2008

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: X, 260

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Pharma

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Buy it now

eBook USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Other ways to access

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