Ribozymes and siRNA Protocols

  • Mouldy Sioud

Part of the Methods in Molecular Biology™ book series (MIMB, volume 252)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Rajesh K. Gaur, Andreas Hanne, Guido Krupp
    Pages 9-17
  3. Kumud K. Singh, Andreas Hanne, Guido Krupp
    Pages 33-48
  4. Dagmar Klein, Camillo Ricordi, Ricardo L. Pastori
    Pages 49-56
  5. Soumitra Basu, Catherine Pazsint, Gopeswar Chowdhury
    Pages 57-75
  6. Hsiu-Hua Chen, Daniela Castanotto, Jeanne LeBon, John J. Rossi, Arthur D. Riggs
    Pages 109-123
  7. Wei-Hua Pan, Gary A. Clawson
    Pages 125-144
  8. Adam Roth, Ronald R. Breaker
    Pages 145-164
  9. Yasuo Komatsu, Eiko Ohtsuka
    Pages 165-177
  10. Emma T. Bowden, Anna T. Riegel
    Pages 179-194
  11. Masayuki Sano, Kazunari Taira
    Pages 195-207
  12. Roger Abounader, Robert Montgomery, Harry Dietz, John Laterra
    Pages 209-219
  13. Jason J. Fritz, Marina Gorbatyuk, Alfred S. Lewin, William W. Hauswirth
    Pages 221-236
  14. Hiroaki Kawasaki, Masaki Warashina, Tomoko Kuwabara, Kazunari Taira
    Pages 237-243
  15. Yoshio Kato, Masaru Tsunemi, Makoto Miyagishi, Hiroaki Kawasaki, Kazunari Taira
    Pages 245-256
  16. Mayu Iyo, Hiroaki Kawasaki, Kazunari Taira
    Pages 257-265
  17. Murray J. Cairns, Lun-Quan Sun
    Pages 267-277

About this book


In this completely updated and expanded edition of a classic bench manual, hands-on experts take advantage of the latest advances in ribozyme, DNAzyme, hammerhead ribozymes and derivatives, and RNA interference technologies to describe in detail the exciting and successful methods now available for gene inactivation in vitro and in vivo. Their optimized techniques employ hairpin ribozymes, DNAzymes, hammerhead ribozymes and derivatives, group I intron ribozymes, RNase P ribozymes, and siRNAs, as well as general methods for RNA structure analysis, delivery of oligonucleotides, and gene therapy. Also provided are novel methods for identifying accessible cellular mRNA sites; group I intron and RNAse P ribozyme protocols for effective design, selection, and therapeutic applications; and the latest RNAi methods for sequence-specific gene silencing in a wide variety of organisms. Additional techniques cover the analysis of ribozyme structures and conformational transitions using nucleotide analog interference mapping and fluorescence resonance energy transfer, the use of ribozymes in clinical and gene therapy, and the use of ribozymes and DNAzymes in rodent models of human disease. Each proven protocol includes a background introduction outlining the principle behind the technique, step-by-step instructions, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and up-to-date, Ribozymes and siRNA Protocols details for experienced and novice investigators alike the many exciting advances in our understanding of nucleic acid enzymes, as well as demonstrating how they may be used to analyze gene function and target validation, and to productively develop novel therapeutics for human diseases.

Editors and affiliations

  • Mouldy Sioud
    • 1
  1. 1.Institute of Cancer ResearchThe Norwegian Radium HospitalOsloNorway

Bibliographic information

  • DOI https://doi.org/10.1385/1592597467
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-226-1
  • Online ISBN 978-1-59259-746-8
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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