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Germ Cell Protocols

Volume 1: Sperm and Oocyte Analysis

  • Heide Schatten

Part of the Methods in Molecular Biology™ book series (MIMB, volume 253)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Hitoshi Sugiyama, Bader Al-Anzi, Robert W. McGaughey, Douglas E. Chandler
    Pages 27-48
  3. Katrina Merkies, Mary M. Buhr
    Pages 49-58
  4. Olufemi A. Olatunbosun, Allison M. Case, Harry G. Deneer
    Pages 95-104
  5. Karim Nayernia, Manyu Li, Wolfgang Engel
    Pages 105-120
  6. Yehudit Lax, Sara Rubinstein, Haim Breitbart
    Pages 135-140
  7. Kathryn J. Mengerink, Victor D. Vacquier
    Pages 141-150
  8. Heng-Yu Fan, Qing-Yuan Sun
    Pages 227-233
  9. Karine Reynaud, Marie Saint-Dizier, Sylvie Chastant-Maillard
    Pages 255-272
  10. Back Matter
    Pages 305-316

About this book

Introduction

Germ cell research has produced enormous advances in recent years and more recently has entered into an explosive phase of new discoveries with the introduction of transgenic technologies and nuclear cloning. In Germ Cell Protocols, expert laboratory investigators describe in step-by-step detail a series of powerful techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Chosen for their proven success in advancing our understanding of germ cells, these methods cover sperm and egg activation, motility, fertilization, nuclear development, nuclear cloning, the molecular characterization of specific events, and the imaging of cell structures. Volume 1: Sperm and Oocyte Analysis focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques. Highlights include in vitro maturation and fertilization of human, porcine, and canine oocytes; the cryopreservation of sperm cells; establishment of an in vitro spermatogenesis system; visualization of sperm accessory structures; and motility assays of spermatozoa. Each readily reproducible protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. An accompanying second volume, Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning, contains methods for molecular egg analysis, live egg imaging, nuclear cloning, oocyte preservation, and nuclear transfer.
Comprehensive and cutting-edge, Germ Cell Protocols offers both novice and established researchers a gold-standard collection of hard-to-find methods for high impact research, diverse procedures that are easy-to-follow, well-illustrated, and allow a cross-species transfer of knowledge from lower vertebrates to higher mammalian systems.

Keywords

Activation DNA cryopreservation electron microscopy genes mammalian oocytes reproducible techniques

Editors and affiliations

  • Heide Schatten
    • 1
  1. 1.Department of Veterinary PathobiologyUniversity of Missouri—ColumbiaColumbia

Bibliographic information

  • DOI https://doi.org/10.1385/1592597440
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-121-9
  • Online ISBN 978-1-59259-744-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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