Germ Cell Protocols

Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning

  • Heide Schatten

Part of the Methods in Molecular Biology™ book series (MIMB, volume 254)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Michele Boiani, James Kehler, Hans R. Schöler
    Pages 1-34
  3. Thiagarajan Venkataraman, Elio Dancausse, Mary L. King
    Pages 67-78
  4. Gisele Höpfl, Max Gassmann, Isabelle Desbaillets
    Pages 79-98
  5. Liangxue Lai, Randall S. Prather
    Pages 149-163
  6. Thomas Rülicke
    Pages 165-194
  7. Yukio Tsunoda, Yoko Kato
    Pages 195-211
  8. Gottfried Brem, Manzoor A. Nowshari
    Pages 213-225
  9. Charles Earle Pope
    Pages 227-244
  10. Mitsuru Naito, Takashi Kuwana
    Pages 245-253
  11. Bruce P. Brandhorst, Graham E. Corley-Smith
    Pages 255-270
  12. Lianchun Fan, Annette Alestrom, Peter Alestrom, Paul Collodi
    Pages 289-299
  13. Steve Pells, Jim McWhir
    Pages 301-311

About this book

Introduction

Germ cell research has produced enormous advances in recent years and more recently has entered into an explosive phase of new discoveries with the introduction of transgenic technologies and nuclear cloning. In Germ Cell Protocols, expert laboratory investigators describe in step-by-step detail a series of powerful techniques for the molecular and genetic analysis of germ cells in a variety of different reproductive systems. Chosen for their proven success in advancing our understanding of germ cells, these methods cover sperm and egg activation, motility, fertilization, nuclear development, nuclear cloning, the molecular characterization of specific events, the imaging of cell structures, and the cryopreservation of germ cells. Volume 2: Molecular Embryo Analysis, Live Imaging, Transgenesis, and Cloning focuses on molecular egg analysis, live egg imaging, nuclear cloning, oocyte cryopreservation, and nuclear transfer. Highlights include the identification and characterization of Oct-4, germline chromatin silencing by RNAi, cDNA subtraction and cloning in the field of trophoblast/placental development, and selective ablation. There are also methods for PCR-based cloning, differentiating embryonic stem cells into embryonic bodies, live imaging of mammalian embryos using multiphoton microscopy, and the cloning of pig, cattle, rabbit, and mouse oocytes. Each readily reproducible protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. An accompanying earlier volume, Sperm and Oocyte Analysis, focuses on sperm cells, oocyte analysis, oocyte maturation, fertilization, and preparation techniques.
Comprehensive and cutting-edge, Germ Cell Protocols offers both novice and established researchers a gold-standard collection of hard-to-find methods for high impact research, diverse procedures that are easy-to-follow, well-illustrated, and allow a cross-species transfer of knowledge from lower vertebrates to higher mammalian systems.

Editors and affiliations

  • Heide Schatten
    • 1
  1. 1.Department of Veterinary PathobiologyUniversity of Missouri-ColumbiaColumbia

Bibliographic information

  • DOI https://doi.org/10.1385/1592597416
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-257-5
  • Online ISBN 978-1-59259-741-3
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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