NMDA Receptor Protocols

  • Min Li

Part of the Methods in Molecular Biology™ book series (MIMB, volume 128)

Table of contents

  1. Front Matter
    Pages i-x
  2. Fumio Nakamura, Yoshio Goshima, Stephen M. Strittmatter, Susumu Kawamoto
    Pages 1-18
  3. Susumu Kawamoto, Satoshi Hattori, Shigeo Uchino, Masayoshi Mishina, Kenji Okuda
    Pages 19-32
  4. Paul L. Chazot, Miroslav Cik, F. Anne Stephenson
    Pages 33-42
  5. Mark A. Varney, Fen-Fen Lin, Christine Jachec, Sara P. Rao, Stephen D. Hess, Edwin C. Johnson et al.
    Pages 43-59
  6. Erik A. Whitehorn, William J. Dower, Min Li
    Pages 61-72
  7. Ronald S. Petralia, Robert J. Wenthold
    Pages 73-92
  8. Ian J. Reynolds, Terre A. Sharma
    Pages 93-102
  9. Su Zhang, Richard L. Huganir
    Pages 103-111
  10. Robert J. Wenthold, Jaroslav Blahos II, Kyung-Hye Huh, Ronald S. Petralia
    Pages 113-119
  11. Stuart A. Lipton
    Pages 121-130
  12. Steven E. Cwirla, William J. Dower, Min Li
    Pages 131-141
  13. Jerry M. Wright, Robert W. Peoples
    Pages 143-153
  14. Hendrika M. A. VanDongen, Antonius M. J. VanDongen
    Pages 155-166
  15. Antonio V. Ferrer-Montiel, Mauricio Montal
    Pages 167-178
  16. Martin Donal Cunningham, Sara Mary Cunningham
    Pages 179-188
  17. Back Matter
    Pages 189-196

About this book

Introduction

In NMDA Receptor Protocols, Min Li and a panel of hands-on experimentalists detail state-of-the-art molecular techniques for studying NMDA ligand-gated ion channels and developing assays for nontherapeutic lead selection. The topics range from cDNA cloning to in vitro and in vivo investigation of the channel complex in the mammalian brain. Additional topics include the biochemical analysis of the channel protein and the construction of various heterologous systems for both basic research and high throughput screens (HTS) for pharmaceutical chemicals. Although the focus is on NMDA receptors, the methods are applicable to other ligand-gated ion channels and with some modification may be extended to related membrane receptors or signaling receptors.
NMDA Receptor Protocols provides today's scientists at all skill levels a diverse collection of highly reproducible molecular techniques for ion channel research and biotherapeutic development. Eminently practical and reproducible, these techniques offer powerful methods for basic research on NMDA receptor structure and function, as well as enormous opportunities for clinical investigation toward the development of novel bioactive compounds.




Editors and affiliations

  • Min Li
    • 1
  1. 1.Johns Hopkins School of MedicineBaltimore

Bibliographic information

  • DOI https://doi.org/10.1385/1592596835
  • Copyright Information Humana Press 1999
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-713-7
  • Online ISBN 978-1-59259-683-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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