Chromatin Protocols

  • Peter B. Becker

Part of the Methods in Molecular Biology™ book series (MIMB, volume 119)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Karolin Luger, Thomas J. Rechsteiner, Timothy J. Richmond
    Pages 1-16
  3. Vasily M. Studitsky
    Pages 17-26
  4. David R. Chafin, Kyu-Min Lee, Jeffrey J. Hayes
    Pages 27-43
  5. Andrew Flaus, Timothy J. Richmond
    Pages 45-60
  6. Ariel Prunell, Mohamed Alilat, Filomena De Lucia
    Pages 79-101
  7. Simon Chandler, Alan P. Wolffe
    Pages 103-112
  8. Jeffrey C. Hansen, Terace M. Fletcher, J. Isabelle Kreider
    Pages 113-125
  9. Jeffrey C. Hansen, Cynthia L. Turgeon
    Pages 127-141
  10. Sanford H. Leuba, Carlos Bustamante
    Pages 143-160
  11. Ralf Erik Wellinger, Renzo Lucchini, Reinhard Dammann, José M. Sogo
    Pages 161-173
  12. Raphael Sandaltzopoulos, Peter B. Becker
    Pages 195-206
  13. Wladyslaw A. Krajewski, Peter B. Becker
    Pages 207-217
  14. Anne-Elisabeth de la Barre, Michel Robert-Nicoud, Stefan Dimitrov
    Pages 219-229
  15. Pierre-Henri Gaillard, Danièle Roche, Geneviève Almouzni
    Pages 231-243
  16. Magdalena Livingstone-Zatchej, Bernhard Suter, Fritz Thoma
    Pages 245-259

About this book

Introduction

More than 40 years after the discovery of the nucleosome as the fun- mental unit of chromatin, the multifaceted problem of how variations in ch- matin structure affect the activity of the eukaryotic genome has not been solved. However, during the past few years research on chromatin structure and fu- tion has gained considerable momentum, and impressive progress has been made at the level of concept development as well as filling in crucial detail. The structure of the nucleosome has been visualized at unprecedented reso- tion. Powerful multisubunit enzymes have been identified that alter histone/ DNA interactions in ways that expose regulatory sequences to factors initi- ing and regulating such nuclear processes as transcription. Though the imp- tance of posttranslational modifications of histones, notably their acetylation, has long been known, the finding that a number of bona fide regulators increase transcription by acetylating nucleosomes has lent new support to the old idea that the process of gene regulation is intimately related to the nature of the chromatin environment. A wealth of nonhistone proteins contribute to a continuum of structures with distinct biochemical properties and varying degrees of DNA condensation. Perhaps the most important conclusion from a large number of studies is a fresh appreciation of the dynamic nature of chromatin structure, the built-in flexibility providing the basis for regulation.

Editors and affiliations

  • Peter B. Becker
    • 1
  1. 1.Adolf-Butenandt-Institut-MolekularbiologieLudwig-Maximilians-UniversitätMünchenGermany

Bibliographic information

  • DOI https://doi.org/10.1385/1592596819
  • Copyright Information Humana Press 1999
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-665-9
  • Online ISBN 978-1-59259-681-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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