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In Situ Hybridization Protocols

  • Book
  • Apr 2014

Overview

Editors:
  1. Ian A. Darby

    1. RMIT University Bundoora, Victoria, Australia

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Part of the book series: Methods in Molecular Biology (MIMB, volume 123)

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Table of contents (21 protocols)

  1. Tissue Techniques

    1. In Situ Hybridization of Whole-Mount Embryos

      • Murray Hargrave, Peter Koopman
      Pages 279-289

    2. cRNA Probes: Comparison of Isotopic and Nonisotopic Detection Methods

      • Teresa Bisucci, Tim D. Hewitson, Ian A. Darby
      Pages 291-303

  2. Applications

    1. Front Matter

      Pages 305-305
      Download chapter PDF

    2. Molecular Cytogenetic Analysis of Sperm from Infertile Males Undergoing Intracytoplasmic Sperm Injection

      • William G. Kearns, Myung-Geol Pang, Darren Griffin, Lesil Brihn, Michael Stacey, Gustavo F. Doncel et al.
      Pages 307-321

    3. Localization of HIV-1 DNA and Tumor Necrosis Factor-α mRNA in Human Brain Using Polymerase Chain Reaction In Situ Hybridization and Immunocytochemistry

      • Steven L. Wesselingh, Jonathan D. Glass
      Pages 323-337

  3. Back Matter

    Pages 339-343
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Keywords

About this book

The technique of in situ hybridization, in its various forms, has been used routinely in many laboratories for a number of years. However, in situ hybridization has retained a reputation as one of the more difficult molecular biological techniques. This may be caused, in part, by the hybrid nature of the technique, which often requires a mixture of molecular biological and histological skills. The two techniques are usually taught and acquired in different streams of biolo- cal science. The step-by-step and detailed protocols provided in this book, by researchers active in the field, should make it possible for both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use the technique successfully in their laboratories. In Situ Hybridization Protocols aims to provide useful protocols for two groups of researchers who use the technique: those who use in situ hybridi- tion to localize genes to particular chromosomes and those researchers who use DNA or RNA probes to localize expression of mRNA in tissue. This new edition also encompasses a number of techniques not covered in the previous edition, such as in situ hybridization of whole-mount embryo specimens, in situ hybridization at the electron microscopic level, and in situ detection of DNA fragmentation in apoptosis. The final two chapters of the book provide examples of application of in situ hybridization techniques to research and clinical problems.

Reviews

From Reviews of the First Edition by Andy Choo. . .

". . .a very helpful manual. . .essential for any laboratory that is using or wants to use the in situ hybridization technique in their repertoire."
--Molecular Biotechnology

" . . .a good book for anyone wishing to exploit all that in situ hybridization has to offer."
-- Biopharm Manufacturing
Reviews for Second Edition...
"This book should be of interest to anyone using or considering in situ hybridization, as it provides well written, if rather specialized, examples of the many applications of this extremely useful but notoriously tricky technique...The text is well referenced and includes useful details of reagent suppliers as well as links to further information available over the internet. This is a book that aims to make in situ hybridization accessible to all, and to inspire new applications for the technique. In my opinion, it does a reasonably sound job of both."-Heredity

"This book is well written and easy to follow and almost all different applications of the in situ hybridization techniques are covered in this volume...highly recommended..." - Cell Biology International

Editors and Affiliations

  • RMIT University Bundoora, Victoria, Australia

    Ian A. Darby

Bibliographic Information

  • Book Title: In Situ Hybridization Protocols

  • Editors: Ian A. Darby

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1385/1592596770

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2000

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XIX, 480

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Pharma

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