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Cytokine Protocols

  • Marc De Ley

Part of the Methods in Molecular Biology book series (MIMB, volume 249)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Peter Härle, Daniel J. J. Carr
    Pages 1-6
  3. Rebecca J. Chan, Min You, Gen-Sheng Feng
    Pages 7-20
  4. Emily Mathey, John Pollard, Patricia Armati
    Pages 41-46
  5. Patricia Menten, Anja Wuyts, Jo Van Damme
    Pages 47-63
  6. Daniela Novick, Menachem Rubinstein
    Pages 65-80
  7. William J. Cook, Mark R. Walter
    Pages 81-92
  8. Sheng-Jiun Wu, Irwin Chaiken
    Pages 93-110
  9. Anthony Meager
    Pages 121-134
  10. Anthony Meager
    Pages 135-151
  11. Anja Wuyts, Patricia Menten, Nancy Van Osselaer, Jo Van Damme
    Pages 153-166
  12. James F. Eliason, Nadine Tare, Sharon Bowen, Tomoaki Inoue, Ikuo Horii
    Pages 167-175
  13. Yong-Yu Liu, Myles C. Cabot
    Pages 177-192
  14. Sandra Hulshof, Danny F. Dukers, Wim Vos, Corline J. A. De Groot
    Pages 193-199
  15. Back Matter
    Pages 239-240

About this book

Introduction

Because cytokines regulate many different bodily functions in living organisms, mainly at the level of cell-cell communications, there is great interest in understanding their mode of action. In Cytokine Protocols, established researchers, physicians, and clinicians present their best biochemical, cellular, and molecular techniques for unraveling and quantifying the events occurring between the initial contact of a cytokine at the membrane receptor and the eventual activation of gene transcription. Described in step-by-step detail to ensure successful experimental results, these protocols cover the large-scale generation and purification of plasmid DNA, the identification of DNA-protein interactions via the gel mobility shift assay, RNA-level phenomena, and the isolation and characterization of cytokines, cytokine-related proteins, and their interactions. The techniques used include the generation of transfectants, the immunohistochemical detection of cytokines in tissue sections, and optimized staining for cytoplasmic detection. Highlights include RT-PCR of small amounts of mRNA, in situ hybridization, biosensor analysis, measurement of biological activities and standardization, immunohistochemical and single-cell detection, and receptor isolation, characterization, and crystallization. Each protocol includes a background introduction, equipment and reagent lists, tips on troubleshooting and avoiding pitfalls, and, where needed, a discussion of the interpretation of results.
Comprehensive and highly practical, Cytokine Protocols offers novice researchers a concise introduction to the principles of cytokine research, and experienced investigators all the advanced techniques and time-saving tips needed to ensure productive research.

Editors and affiliations

  • Marc De Ley
    • 1
  1. 1.Laboratory of Biochemistry, Department of ChemistryKatholieke Universiteit LeuvenLeuvenBelgium

Bibliographic information

  • DOI https://doi.org/10.1385/1592596673
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-948-3
  • Online ISBN 978-1-59259-667-6
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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