Drosophila Cytogenetics Protocols

  • Daryl S. Henderson

Part of the Methods in Molecular Biology book series (MIMB, volume 247)

Table of contents

  1. Front Matter
    Pages i-xiii
  2. Daryl S. Henderson
    Pages 1-43
  3. Helen White-Cooper
    Pages 45-75
  4. Endre Máthé
    Pages 89-127
  5. Seppo Nokkala, Christina Nokkala
    Pages 129-137
  6. Dmitry E. Koryakov, Natalia I. Maľceva, Robert C. King, Igor F. Zhimulev
    Pages 139-161
  7. Kristen M. Johansen, Jørgen Johansen
    Pages 215-234
  8. Adelaide T. C. Carpenter
    Pages 257-277
  9. Robert D. C. Saunders
    Pages 279-287
  10. Sergey Lavrov, Jérôme Déjardin, Giacomo Cavalli
    Pages 289-303
  11. Valery F. Semeshin, Elena S. Belyaeva, Victor V. Shloma, Igor F. Zhimulev
    Pages 305-324
  12. Laura Fanti, Sergio Pimpinelli
    Pages 325-332
  13. Laura Fanti, Sergio Pimpinelli
    Pages 353-361

About this book

Introduction

Probably no single experimental organism has contributed as much to our understanding of the structure, function, and mechanism of transmission of eukaryotic chromosomes as has the fruit fly, Drosophila melanogaster. In Drosophila Cytogenetics Protocols, leading drosophilists describe, in step-by-step detail, all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. General areas covered include analyses of nuclei and chromosomes in spermatogenesis and oogenesis, fluorescence-activated cell sorting of ovarian follicle cells, analyses of nuclear division cycles in embryos, and analyses of programmed cell death. In addition, there are conventional light-, electron-, and immunofluorescence-microscopic analyses of polytene chromosomes; cytological analyses of larval brains, imaginal discs, and histoblasts; and nonfluorescent and fluorescent in situ hybridization (FISH) to both polytene and mitotic chromosomes. A cutting-edge combined FISH and immunolocalization protocol will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression. Each readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls.
Readily accessible and highly practical, Drosophila Cytogenetics Protocols provides a comprehensive cytogenetics laboratory manual for Drosophila investigators, one that is suitable not only for novices, but also highly informative for seasoned investigators.

Keywords

DNA FISH electron microscopy gene expression hybridization

Editors and affiliations

  • Daryl S. Henderson
    • 1
  1. 1.Department of Pharmacological SciencesState University of New York at Stony Brook

Bibliographic information

  • DOI https://doi.org/10.1385/1592596657
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-050-2
  • Online ISBN 978-1-59259-665-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book