Gene Delivery to Mammalian Cells

Volume 1: Nonviral Gene Transfer Techniques

  • William C. Heiser

Part of the Methods in Molecular Biology™ book series (MIMB, volume 245)

Table of contents

  1. Front Matter
    Pages i-xv
  2. Delivery Using Chemical Methods

    1. Front Matter
      Pages 1-1
    2. Dexi Liu, Evelyn F. Chia, Hui Tian
      Pages 3-23
    3. Lei Zhang, Nicholas Ambulos, A.James Mixson
      Pages 33-52
    4. Todd D. Giorgio, Shelby K. Wyatt
      Pages 53-65
    5. James R. Baker Jr., Anna U. Bielinska, Jolanta F. Kukowska-Latallo
      Pages 67-81
    6. Shelby K. Wyatt, Todd D. Giorgio
      Pages 83-93
    7. John Marshall, Nelson S. Yew, Seng H. Cheng
      Pages 95-114
    8. Young K. Song, Guisheng Zhang, Dexi Liu
      Pages 115-124
    9. Duen-Hwa Yan, Bill Spohn, Mien-Chie Hung
      Pages 125-135
  3. Delivery Using Physical Methods

    1. Front Matter
      Pages 145-145
    2. Te-hui W. Chou, Subhabrata Biswas, Shan Lu
      Pages 147-165
    3. Shixia Wang, Swati Joshi, Shan Lu
      Pages 185-196
    4. Nathalie Dujardin, Véronique Préat
      Pages 215-226

About this book

Introduction

Whether to assess the function of new genes identified from the Human Genome Project or to apply gene therapy successfully, it is often necessary to deliver genes to specific cells. In Gene Delivery to Mammalian Cells, highly experienced researchers describe in great detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1: Nonviral Gene Transfer Techniques focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, and hydrodynamics. Each protocol contains step-by-step instructions, along with background notes, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. Introductory chapters review the delivery methods presented, discussing their advantages and disadvantages, how they have been used successfully for gene delivery, and the future of their technology. An accompanying volume, Volume 2: Viral Gene Transfer Techniques, details procedures for delivering genes to cells in vitro and in vivo.
Comprehensive and cutting-edge, Gene Delivery to Mammalian Cells, volumes 1 and 2, offers biomedical specialists in industry and academia an unrivaled collection of reliable methods for the chemical, physical, and viral delivery of genes to mammalian cells, all prepared to save laboratory time and ensure experimental success.

Keywords

DNA Ethylen Vivo gene transfer genes liposomes

Editors and affiliations

  • William C. Heiser
    • 1
  1. 1.Bio-Rad LaboratoriesHercules

Bibliographic information

  • DOI https://doi.org/10.1385/1592596495
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-086-1
  • Online ISBN 978-1-59259-649-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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