Overview
- Editors:
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Mark Doolittle
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University of California and West Los Angeles VA Medical Center, Los Angeles
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Karen Reue
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University of California and West Los Angeles VA Medical Center, Los Angeles
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Table of contents (30 protocols)
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Lipase Activity, Purification, and Expression
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- Sofiane Bezzine, Francine Ferrato, Véronique Lopez, Alain de Caro, Robert Verger, Frédéric Carrière
Pages 187-202
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- Stéphane Canaan, Liliane Dupuis, Mireille Rivière, Robert Verger, Catherine Wicker-Planquart
Pages 203-213
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Biochemical Characterization
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- Mark H. Doolittle, Osnat Ben-Zeev
Pages 215-237
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- Alain De Caro, Sofiane Bezzine, Véronique Lopez, Mustapha Aoubala, Cécile Daniel, Robert Verger et al.
Pages 239-256
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- Osnat Ben-Zeev, Mark H. Doolittle
Pages 257-266
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- Stéphane Ransac, Margarita Ivanova, Ivan Panaiotov, Robert Verger
Pages 279-302
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Molecular Genetic Characterization
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- Gouri Ranganathan, Philip A. Kern
Pages 329-335
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- Gouri Ranganathan, Philip A. Kern
Pages 337-341
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Back Matter
Pages 357-362
About this book
The lipases and phospholipases represent a diverse group of enzymes that are expressed in animals, plants, fungi, and bacteria. Their ubiquitous distribution among all species is a testament to the essential roles played by these enzymes in lipid storage, mobilization and metabolism, membrane homeostasis and remodeling, endocrine and immune functions, and signal tra- duction. In humans, lipases and phospholipases are also thought to contribute to complex diseases, such as atherosclerosis, obesity, arthritis, and cancer, as well as to single gene defects, such as Wolman's disease and Type I hyperlipoproteinemia. Enzymatically, the lipases are unique, since they hydrolyze substrates that are either insoluble, or only partly soluble, in aq- ous solvents; thus, enzyme catalysis takes place at a lipid-water interface. The interface comprises at least two, and often more, discrete bulk and s- face phases, in which the enzyme, substrate, and products oflipolysis disperse among these phases based on their physical properties. Furthermore, the d- tribution of these components changes continuously as lipolysis proceeds. Thus, the lipases and phospholipases are fundamentally different from any other enzyme because of the physical complexity of the environment in which catalysis occurs.
Reviews
"...The book can be recommended to those scientists who are deeply engaged in lipid chemistry and analysis as well as enzymology. It provides a detailed listing of literature and other sources. Thus it can be successfully used as an introduction to new research fields and experimental techniques..."-Nahrung
"In view of the rapid increase in the number of new lipases and phospholipases that have been recently identified, the focus of this book is timely...In this volume, modern, up-to-date protocols with sufficient detail for experienced or peripheral investigators in this area are provided, and should prove to be a valuable resource."-The Quarterly Review of Biology
