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Mammalian Artificial Chromosomes

Methods and Protocols

  • Vittorio Sgaramella
  • Sandro Eridani

Part of the Methods in Molecular Biology book series (MIMB, volume 240)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Vittorio Sgaramella, Sandro Eridani
    Pages 1-13
  3. Gérard Roizés, Christoph Grunau, Jérôme Buard, Albertina De Sario, Jacques Puechberty
    Pages 77-104
  4. Alessandro Vindigni, Gulnara Abdurashidova, Arturo Falaschi
    Pages 105-122
  5. Fiorentina Ascenzioni, Pier Assunta Fradiani, Pierluigi Donini
    Pages 123-146
  6. Han N. Lim, Christine J. Farr
    Pages 167-186
  7. Jose I. de las Heras, Leonardo D’Aiuto, Howard Cooke
    Pages 187-206
  8. Yoshimi Kuroiwa, Kazuma Tomizuka, Isao Ishida
    Pages 207-225
  9. Diane P. Monteith, Josephine D. Leung, Anita H. Borowski, Deborah O. Co, Tünde Praznovszky, Frank R. Jirik et al.
    Pages 227-242
  10. Michael R. Orford, Duangporn Jamsai, Samuel McLenachan, Panayiotis A. Ioannou
    Pages 243-266
  11. Back Matter
    Pages 267-269

About this book

Introduction

Assembly of the basic units of mammalian chromosomes into artificial constructs, and the reduction of their components to more manageable dimensions, has now become a reality. In Mammalian Artificial Chromosomes: Methods and Protocols, leading scientists in cell biology, genetics, cell culturing, and gene transfer present their most productive methods for the preparation, characterization, and use of artificial chromosomes. The authors cover all relevant areas of artificial chromosome research-from basic genetics to daring attempts at building new gene therapy tools-and describe possible applications in genetic manipulation requiring large portions of the genome. Each fully tested protocol is described in step-by-step detail and includes a background introduction outlining the principle behind the technique, reagent and equipment lists, and tips on troubleshooting and how to avoid pitfalls.
Cutting-edge and much-needed, Mammalian Artificial Chromosomes: Methods and Protocols offers a timely collection of meticulous techniques that will help elucidate the mechanisms of nuclear duplication, allow the introduction of changes into the human genome, and revolutionize our understanding of how chromosomes work.

Editors and affiliations

  • Vittorio Sgaramella
    • 1
  • Sandro Eridani
    • 1
  1. 1.Istituto di Tecnologie Biomediche AvanzateConsiglio Nationale RicercheSegrateItaly

Bibliographic information

  • DOI https://doi.org/10.1385/1592594344
  • Copyright Information Humana Press 2004
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-096-0
  • Online ISBN 978-1-59259-434-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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