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p53 Protocols

  • Sumitra Deb
  • Swati Palit Deb

Part of the Methods in Molecular Biology book series (MIMB, volume 234)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Matthew Holmes, Elizabeth Rosenberg, Kristoffer Valerie
    Pages 1-16
  3. Xiu Zhu Sun, John Nguyen, Jamil Momand
    Pages 17-28
  4. Melissa L. Dumble, Lawrence A. Donehower, Xiongbin Lu
    Pages 29-49
  5. Susanne Stein, Yong-Jig Cho, Roger S. Jackson II, Peng Liang
    Pages 51-63
  6. Joanna K. Sax, Wafik S. El-Deiry
    Pages 65-71
  7. Mariano J. Scian, Rebecca Frum, Swati Palit Deb, Sumitra Deb
    Pages 93-110
  8. Patrick Dumont, Anthony Della Pietra, Maureen E. Murphy
    Pages 111-120
  9. David M. Keller, Shelya X. Zeng, Hua Lu
    Pages 121-133
  10. Judith Roth, Matthias Dobbelstein
    Pages 135-149
  11. Maria Patricia Molina, Christine Cain, Jill Bargonetti
    Pages 151-170
  12. Ashley L. Craig, Susan E. Bray, Lee E. Finlan, Neil M. Kernohan, Ted R. Hupp
    Pages 171-202
  13. Motohiro Mihara, Ute M. Moll
    Pages 203-209
  14. Susan Erster, Neda Slade, Ute M. Moll
    Pages 219-230
  15. Neda Slade, Ute M. Moll
    Pages 231-243
  16. Michael Berger, Ygal Haupt
    Pages 245-256
  17. Rebecca Frum, Swati Palit Deb
    Pages 257-267
  18. Back Matter
    Pages 269-280

About this book

Introduction

Since the discovery of p53 as a tumor suppressor, numerous methods have evolved to reveal its unique structural features and biochemical functions. In p53 Protocols, Sumitra and Swati Palit Deb have assembled an indispensable collection of novel techniques that have proven most useful for studying the physiological properties of p53 both in vitro and in vivo. Described by leading basic and clinical researchers who have successfully used the methods, the techniques provide proven solutions to problems in studying the purification, target identification, gene expression, quantitation, interaction, signaling, transactivation, and transrepression of p53. The methods are also useful for delineating the functions of other proteins that may act as tumor or growth suppressors. Each technique includes step-by-step instructions, troubleshooting notes, a theoretical review, and discussion of associated problems that might arise during the course of investigation. Special effort has been made to discuss problems and their solutions to help ease the learning curve when standardizing a new method.
Diverse and highly practical, p53 Protocols offers both beginning and experienced researchers in cancer biology a gold-standard compendium of readily reproducible techniques for studying p53 and related proteins.

Editors and affiliations

  • Sumitra Deb
    • 1
  • Swati Palit Deb
    • 1
  1. 1.Medical College of VirginiaVirginia Commonwealth UniversityRichmond

Bibliographic information

  • DOI https://doi.org/10.1385/1592594085
  • Copyright Information Humana Press 2003
  • Publisher Name Springer, Totowa, NJ
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-106-6
  • Online ISBN 978-1-59259-408-5
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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