Vaccine Protocols

  • Andrew Robinson
  • Michael J. Hudson
  • Martin P. Cranage

Part of the Methods in Molecular Medicine™ book series (MIMM, volume 87)

Table of contents

  1. Front Matter
    Pages i-xi
  2. Gordon Ada
    Pages 1-17
  3. Craig R. Pringle
    Pages 19-36
  4. Anthony R. Fooks
    Pages 37-49
  5. Caroline Staib, Gerd Sutter
    Pages 51-68
  6. Gunilla B. Karlsson, Peter Liljeström
    Pages 69-81
  7. Frances Bowe, Derek J. Pickard, Richard J. Anderson, Patricia Londoño-Arcila, Gordon Dougan
    Pages 83-100
  8. Mark A. Reuter, Sean Hanniffy, Jerry M. Wells
    Pages 101-114
  9. Michael J. Francis
    Pages 115-131
  10. Mariagrazia Pizza, Maria Rita Fontana, Vincenzo Scarlato, Rino Rappuoli
    Pages 133-151
  11. Carla C. A. M. Peeters, Patrick R. Lagerman, Odo de Weers, Lukas A. Oomen, Peter Hoogerhout, Michel Beurret et al.
    Pages 153-173
  12. Duncan E. S. Stewart-Tull
    Pages 175-193
  13. Linda S. Klavinskis, Philip Hobson, Andrew Woods
    Pages 195-210
  14. David H. Jones
    Pages 211-222
  15. Gerald D. J. Adams
    Pages 223-243
  16. David J. M. Lewis, Christopher M. M. Hayward
    Pages 245-253
  17. Kingston H. G. Mills
    Pages 255-277
  18. Ray Borrow, Paul Balmer
    Pages 289-300
  19. Nigel J. Saunders, Sarah Butcher
    Pages 301-312

About this book


This extensively revised, updated, and expanded second edition of a classic laboratory manual applies new understanding of molecular mechanisms to the rational design and development of vaccines. Here the working researcher will find readily reproducible techniques for the development and production of live attenuated vaccines through the genetic manipulation of viruses and bacteria, vaccine vectors or inactivated toxins, and the production of synthetic peptides and conjugate vaccines. In addition, there are chapters describing more general techniques for vaccine formulation, delivery, and assessment of immune responses, and review discussions of scale-up to manufacture, vaccine quality assurance, and clinical trials. New to this second edition are cutting-edge chapters on two different viral vectors (Vaccinia and Semliki Forest), lactic acid bacteria for delivery of foreign antigens, incorporation of immunomodulators into plasmid DNA vaccines, construction of tetramer complexes to detect CMI responses, assessment of functional antibody responses, and the use of complete genome sequences for vaccine design. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.
State-of-the-art and highly practical, Vaccine Protocols, Second Edition, provides essential information for those seeking to produce improved or novel vaccines in the rapidly evolving field of vaccine research and development.

Editors and affiliations

  • Andrew Robinson
    • 1
  • Michael J. Hudson
    • 1
  • Martin P. Cranage
    • 2
  1. 1.Centre for Applied Microbiology and ResearchHealth Protection AgencySalisburyUK
  2. 2.Department of Cellular and Molecular MedicineSt. George’s Hospital Medical SchoolLondonUK

Bibliographic information

  • DOI
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-140-0
  • Online ISBN 978-1-59259-399-6
  • Series Print ISSN 1543-1894
  • Series Online ISSN 1940-6037
  • About this book
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