Protein Kinase C Protocols

  • Alexandra C. Newton

Part of the Methods in Molecular Biology™ book series (MIMB, volume 233)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Introduction

    1. Front Matter
      Pages 1-1
    2. Alexandra C. Newton
      Pages 3-7
    3. Yasutomi Nishizuka, Ushio Kikkawa
      Pages 9-18
  3. Expression and Purification

    1. Front Matter
      Pages 19-19
    2. Hideyuki Mukai, Yoshitaka Ono
      Pages 21-34
    3. Michael Gschwendt, Luise Stempka
      Pages 35-42
  4. Measuring the Activity of Protein Kinase C

    1. Front Matter
      Pages 43-43
    2. Julianne J. Sando
      Pages 45-61
    3. Julianne J. Sando, Jacquelyn K. Beals
      Pages 63-76
    4. Julianne J. Sando, Jacquelyn K. Beals, Isa M. Hussaini
      Pages 77-85
  5. Measuring the Interaction of Protein Kinase C with Membranes

  6. Measuring the Phosphorylation of Protein Kinase C

    1. Front Matter
      Pages 157-157
    2. Peter J. Parker
      Pages 159-162
    3. Mikiko Takahashi, Yoshitaka Ono
      Pages 163-170

About this book

Introduction

Protein kinase C impacts on nearly every aspect of signal transduction, placing it at the forefront of research in how cells transduce information. In Protein Kinase C Protocols, leading investigators from academia and industry present a comprehensive collection of classical and emerging laboratory techniques for the study of this influential protein, all optimized for successful and reproducible results. Described in step-by-step detail, these methods can be easily used to explore the structure, function, regulation, subcellular localization, and macromolecular interactions of protein kinase C. Included in this collection are easy-to-follow techniques to purify protein kinase C, to measure its activity and its membrane interaction in vivo and in vitro, and to identify substrates. There are also methodologies and techniques for measuring the phosphorylation state of protein kinase C, for expressing and analyzing the membrane-targeting domains of protein kinase C, and for detecting binding proteins. Additional chapters address pharmacological probes, genetic approaches, and protein kinase C in disease. Each protocol is introduced in the context of protein kinase C function and regulation and contains many notes on how best to deal with the problems that may occur.
Comprehensive and authoritative, Protein Kinase C Protocols offers a timely compilation of biophysical, biochemical, cell biological, and molecular biological approaches that will ensure successful results in every laboratory working in this rapidly advancing field.

Editors and affiliations

  • Alexandra C. Newton
    • 1
  1. 1.Department of PharmacologyUniversity of California at San DiegoLa Jolla

Bibliographic information

  • DOI https://doi.org/10.1385/1592593976
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-068-7
  • Online ISBN 978-1-59259-397-2
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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