Functional Genomics

Methods and Protocols

  • Michael J. Brownstein
  • Arkady B. Khodursky

Part of the Methods in Molecular Biology book series (MIMB, volume 224)

Table of contents

  1. Front Matter
    Pages i-xii
  2. Charlie C. Xiang, Michael J. Brownstein
    Pages 1-7
  3. George Jokhadze, Stephen Chen, Claire Granger, Alex Chenchik
    Pages 9-29
  4. Alexander Munishkin, Konrad Faulstich, Vissarion Aivazachvili, Claire Granger, Alex Chenchik
    Pages 31-54
  5. Charlie C. Xiang, Michael J. Brownstein
    Pages 55-60
  6. Arkady B. Khodursky, Jonathan A. Bernstein, Brian J. Peter, Virgil Rhodius, Volker F. Wendisch, Daniel P. Zimmer
    Pages 61-78
  7. Gordon K. Smyth, Yee Hwa Yang, Terry Speed
    Pages 111-136
  8. Asa Ben-Hur, Isabelle Guyon
    Pages 159-182
  9. Ying Zhao, George Karypis
    Pages 183-218
  10. Gavin Sherlock, Catherine A. Ball
    Pages 235-248
  11. Back Matter
    Pages 249-258

About this book

Introduction

As high-throughput methods become more available and routine, experimentalists and theoreticians must be prepared to take advantage of the analytical power these methods offer. In Functional Genomics: Methods and Protocols, leading experimentalists-many pioneers in the field-describe in step-by-step detail robust methods for microarray-based studies and provide expert guidance in optimal data analysis and informatics. Drawing on insights derived from their long experience in biology, neurobiology, cell biology, molecular biology, and statistics, the authors review each of the key methods and analytical techniques used in functional genomics. On the methods side, this includes proven techniques for monitoring subcellular RNA localization en masse, for mapping chromosomes at the resolution of a single gene, and for surveying the steady-state genome-wide distribution of DNA binding proteins in vivo. For those workers dealing with massive data sets, the book discusses the methodological aspects of data analysis and informatics in the design of microarray experiments, the choice of test statistic, and the assessment of observational significance, data reduction, and clustering. Additional chapters outline the many available approaches to data visualization and storage so crucial to the successful mining and examination of experimental results.
Timely and cutting-edge, Functional Genomics: Methods and Protocols offers novice and advanced investigators in all biological fields a collection of highly practical, readily reproducible methods, data analysis techniques, and experimental design criteria that will ensure safe passage through one of the most demanding and fastest growing fields today.

Editors and affiliations

  • Michael J. Brownstein
    • 1
  • Arkady B. Khodursky
    • 2
  1. 1.Laboratory of Genetics, NIMH/NHGRINational Institutes of HealthRockville
  2. 2.Department of Biochemistry, Molecular Biology and BiophysicsUniversity of MinnesotaSt. Paul

Bibliographic information

  • DOI https://doi.org/10.1385/159259364X
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-1-58829-291-9
  • Online ISBN 978-1-59259-364-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • About this book
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