Skip to main content
  • Book
  • © 2002

Transgenic Mouse Methods and Protocols

Part of the book series: Methods in Molecular Biology (MIMB, volume 209)

Buying options

eBook USD 79.99
Price excludes VAT (USA)
  • ISBN: 978-1-59259-340-8
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout

This is a preview of subscription content, access via your institution.

Table of contents (18 protocols)

  1. Front Matter

    Pages i-xiii
  2. Genetic Modification of the Mouse

    • J. Willem Voncken
    Pages 9-34
  3. In Vitro Fertilization/Cryopreservation

    • Susan Marschall, Martin Hrabě de Angelis
    Pages 35-50
  4. Transgene Design

    • J. Willem Voncken
    Pages 51-67
  5. Manipulating Large Insert Clones for Transgenesis

    • Sally P. A. McCormick, Catherine Y. Y. Liu, Stephen G. Young, Lars B. Nielsen
    Pages 105-123
  6. Reporter Molecules in Genetically Engineered Mice

    • Thomas L. Saunders
    Pages 125-143
  7. Gene Targeting in Mouse Embryonic Stem Cells

    • Jan van Deursen
    Pages 145-158
  8. Conditional Knockout Mice

    • Ralf Kühn, Frieder Schwenk
    Pages 159-185
  9. Knockin Approaches

    • Anton J. M. Roebroek, Xiaosheng Wu, Richard J. Bram
    Pages 187-200
  10. Aggregation of Embryos and Embryonic Stem Cells

    • Ronald Maatman, Marina Gertsenstein, Emile de Meijer, Andras Nagy, Kristina Vintersten
    Pages 201-230
  11. Andenovirus-Mediated Gene Transfer

    • Ko Willems van Dijk, Kyriakos E. Kypreos, Christine d’Oliveira, Frits J. Fallaux
    Pages 231-247
  12. Random ENU Mutagenesis

    • Dian Soewarto, Véronique Blanquet, Martin de Hrabě Angelis
    Pages 249-266
  13. Autopsy and Histologic Analysis of the Transgenic Mouse

    • Marion J. J. Gijbels, Menno P. J. de Winther
    Pages 267-279
  14. Bone Marrow Transplantations to Study Gene Function in Hematopoietic Cells

    • Menno P. J. de Winther, Peter Heeringa
    Pages 281-292
  15. Quantification of Atherosclerosis in Mice

    • Alan Daugherty, Stewart C. Whitman
    Pages 293-309
  16. Genetically Modified Mice in Cancer Research

    • David A. Largaespada
    Pages 311-332
  17. Neuropathobiology in Transgenic Mice

    • Jo Van Dorpe, Kurt Spittaels, Chris Van den Haute, Ilse Dewachter, Dieder Moechars, Hugo Geerts et al.
    Pages 333-361
  18. Back Matter

    Pages 363-374

About this book

Genetically very similar to the human species, mice play an important role in biomedical research and have served as experimental models for a wide variety of pathologies, including cancer, cardiovascular diseases, and behavioral disorders. In Transgenic Mouse Methods and Protocols, Marten Hofker and Jan van Deursen have assembled a multidisciplinary collection of readily reproducible methods for working with mice, and particularly for generating mouse models that will enable us to better understand gene function. Described in step-by-step detail by highly experienced investigators, these proven techniques include new methods for conditional, induced knockout, and transgenic mice, as well as for working with mice in such important research areas as immunology, cancer, and atherosclerosis. Such alternative strategies as random mutagenesis and viral gene transduction for studying gene function in the mouse are also presented. Care is taken to make clear the details of the available approaches, as well as their limitations.
Up-to-date and highly practical, Transgenic Mouse Methods and Protocols demonstrates clearly for both novice and expert investigators how to make novel transgenic mouse models, and how to use them effectively to understand the role of gene function in human health and disease.


"...highly recommendable to cyto- and histochemist researchers, since they can easily find the way to enrich the instrumentarium of their own conceptual tools." - European Journal of Histochemistry

Editors and Affiliations

  • Department of Molecular Genetics, University of Maastricht, Maastricht, The Netherlands

    Marten H. Hofker

  • Department of Pediatrics and Adolescent Medicine, Mayo Clinic, Rochester, MN

    Jan Deursen

Bibliographic Information

  • Book Title: Transgenic Mouse Methods and Protocols

  • Editors: Marten H. Hofker, Jan Deursen

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana Totowa, NJ

  • eBook Packages: Springer Protocols

  • Copyright Information: Humana Press 2002

  • eBook ISBN: 978-1-59259-340-8

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: 392

  • Number of Illustrations: 76 b/w illustrations, 4 illustrations in colour

  • Topics: Cell Biology

  • Industry Sectors: Biotechnology, Health & Hospitals, Pharma

Buying options

eBook USD 79.99
Price excludes VAT (USA)
  • ISBN: 978-1-59259-340-8
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout