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Capillary Electrophoresis of Carbohydrates

  • Pierre Thibault
  • Susumu Honda

Part of the Methods in Molecular Biology™ book series (MIMB, volume 213)

Table of contents

  1. Front Matter
    Pages i-xiv
  2. Introduction

    1. Front Matter
      Pages 1-1
  3. Sample Preparation Methods

    1. Front Matter
      Pages 25-25
    2. Tony Merry, Sviatlana Astrautsova
      Pages 27-40
    3. Shigeo Suzuki, John F. Kelly, Steven J. Locke, Pierre Thibault, Susumu Honda
      Pages 41-69
  4. Analysis of Mono- and Oligosaccharides

  5. Analysis of Glycoconjugates and the Component Monosaccharides

    1. Front Matter
      Pages 145-145
    2. Myriam Taverna, Nguyet Thuy Tran, Danielle Ferrier
      Pages 163-196
    3. Ira S. Krull, Sarah Kazmi, Huijuan Zhong, Ling Chen Santora
      Pages 197-218
    4. Kevin P. Bateman, John F. Kelly, Pierre Thibault, Louis Ramaley, Robert L. White
      Pages 219-239
    5. Pierre Thibault, Adèle Martin, Michel Gilbert, Warren W. Wakarchuk, James C. Richards
      Pages 241-259
  6. Specific Applications

    1. Front Matter
      Pages 261-261
    2. Warren W. Wakarchuk, Anna-Maria Cunningham
      Pages 263-274
  7. Back Matter
    Pages 285-317

About this book

Introduction

Recent developments in high-resolution separation techniques based on capillary-scale chromatography and electrophoresis have transformed the analysis of free and conjugated mono- and oligosaccharides. In Capillary Electrophoresis of Carbohydrates, hands-on experts describe cutting-edge techniques in capillary electrophoresis (CE) for the analysis of complex carbohydrates. Written in step-by-step detail to ensure successful experimental results, these readily reproducible protocols provide methods for sample preparation and analysis of mono- and oligosaccharides, glycoproteins, and glycoconjugates. Different CE separation formats, including capillary zone electrophoresis (CZE), capillary isoelectric focusing (CIEF), capillary isotachophoresis (CITP), capillary gel electrophoresis (CGE), and micellar electrokinetic chromatography (MEKC), are presented for different types of applications, such as the analysis of glycoforms, the separation of enantiomers, and the resolution of oligosaccharides. The separation of native derivatized carbohydrates is presented using UV, fluorescence, and mass spectrometric detection. Two other applications demonstrate the use of CE as an assay method for monitoring glycosyltransferase activity and for determining the association constant of carbohydrate-protein interaction. A useful appendix describes the structures of the most commonly encountered carbohydrate residues and oligosaccharides from mammalian and bacterial origins. Each protocol contains detailed information on reagents, apparatus, and valuable comments and notes on methods.
Timely and highly practical, Capillary Electrophoresis of Carbohydrates provides both novice and experienced CE analysts with powerful tools for the successful separation of complex carbohydrates by capillary electrophoresis.

Editors and affiliations

  • Pierre Thibault
    • 1
  • Susumu Honda
    • 2
  1. 1.Caprion PharmaceuticalsMontrealCanada
  2. 2.Faculty of Pharmaceutical SciencesKinki UniversityJapan

Bibliographic information

  • DOI https://doi.org/10.1385/1592592945
  • Copyright Information Humana Press 2003
  • Publisher Name Humana Press
  • eBook Packages Springer Protocols
  • Print ISBN 978-0-89603-826-4
  • Online ISBN 978-1-59259-294-4
  • Series Print ISSN 1064-3745
  • Series Online ISSN 1940-6029
  • Buy this book on publisher's site
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